Bioconjugates and delivery of bioactive agents

ABSTRACT

The present invention relates to bioconjugates and the delivery of bioactive agents which are preferably targeted for site-specific release in cells, tissues or organs. More particularly, this invention relates to bioconjugates which comprise a bioactive agent and an organocobalt complex. The bioactive agent is covalently bonded directly or indirectly to the cobalt atom of the organocobalt complex. The bioactive agent is released from the bioconjugate by the cleavage of the covalent bond between the bioactive agent and the cobalt atom in the organocobalt complex. The cleavage may occur as a result of normal displacement by cellular nucleophiles or enzymatic action, but is preferably caused to occur selectively as a predetermined release site by application of an external signal. The external signal may be light or photoexcitation, i.e. photolysis, or it may be ultrasound, i.e. sonolysis. Further, if the photolysis takes place in the presence of a magnetic field surrounding the release site, the release of the bioactive agent into surrounding healthy tissue is minimized.

The present application is a divisional application of U.S. patentapplication Ser. No. 09/202,328 filed on 22 Oct. 1999, now U.S. Pat. No.6,315,978, which is a national stage filing under 35 U.S.C. §371 ofPCT/US97/14140 filed on 22 Aug. 1997. The present application is furtherrelated to U.S. provisional patent applications Serial No. 60/024,430filed on 27 Aug. 1996 and Serial No. 60/025,036 filed on 27 Aug. 1996,to which priority is claimed under 35 U.S.C. §119(e).

This invention was made in part with Government support under Grant No.ES05728 awarded by the National Institutes of Health, Bethesda, Md. TheUnited States Government has certain rights in the invention.

BACKGROUND OF THE INVENTION

The present invention relates to bioconjugates and the delivery ofbioactive agents which are preferably targeted for site-specific releasein cells, tissues or organs. More particularly, this invention relatesto bioconjugates which comprise a bioactive agent and an organocobaltcomplex. The bioactive agent is covalently bonded directly or indirectlyto the cobalt atom of the organocobalt complex. The bioactive agent isreleased from the bioconjugate by the cleavage of the covalent bondbetween the bioactive agent and the cobalt atom in the organocobaltcomplex. The cleavage may occur as a result of normal displacement bycellular nucleophiles or enzymatic action, but is preferably caused tooccur selectively at a predetermined release site by application of anexternal signal. The external signal may be light or photoexcitation,i.e. photolysis, or it may be ultrasound, i.e. sonolysis. Further, ifthe photolysis takes place in the presence of a magnetic fieldsurrounding the release site, the release of the bioactive agent intosurrounding healthy tissue is minimized.

The publications and other materials used herein to illuminate thebackground of the invention, and in particular, cases to provideadditional details respecting the practice, are incorporated byreference, and for convenience are referenced in the following text byauthor and date and are listed alphabetically by author in the appendedbibliography.

The focus of a substantial body of research has been the development ofa system whereby a pharmaceutical agent can be selectively delivered toa desired anatomic location; namely the site in need of treatment. Inspite of the great progress which has been achieved in this regard, manypharmaceutical delivery systems for the treatment of various diseases orhealth risks, e.g., the treatment of cancer, impart substantial risk tothe patient. With respect to the treatment of cancer, drugs which areeffective in attacking malignant cells to destroy them, or at leastlimit their proliferation, have a tendency to attack benign cells also.Therefore, it is highly desirable to limit the location of their actionto that of the malignancy, and to ensure that at any particular timeeffective, but not excessive, amounts of such drugs are used.

Although it is desired to concentrate a cytotoxic agent at a targetedsite, current cancer treatment protocols for administering thesecytotoxic agents typically call for repeated intravenous dosing, withcareful monitoring of the patient. The drugs are often used incombination to exert a multi-faceted assault on neoplastic cells. Thedose is selected to be just below the amount that will produce acute(and sometimes chronic) toxicity that can lead to life-threateningcardiomyopathy, myelotoxicity, hepatic toxicity, or renal toxicity.Alopecia (hair loss), mucositis, stomatitis, and nausea are othercommon, but generally not life-threatening, side effects at these doses.Since many of these compounds are potent vesicants, tissue necrosis willoccur if localized extravasation (loss of the drug from blood to thesurrounding tissue) occurs. These effects occur since the bloodgenerally attains a specified concentration of that drug before becomingeffective. Because the blood is transported throughout the body of thehost being treated, so is the pharmaceutical agent. Following thistechnique provides an even distribution of the drug throughout the body,rather than concentrating it at the treatment site. Moreover, suchsystemic treatment methods expose the healthy cells to the cytotoxicagent concurrent with the treatment of the unhealthy or diseased cellsbesides limiting the concentration of the drug at the site where it ismost needed.

Previous attempts to administer such drugs by direct injection into thelocation of the organ having the malignancy are only partiallyeffective, because of migration of the drug from that location and as aresult of extensive tissue necrosis from extravasation. Such dispersioncannot be totally prevented, with the result that excessive quantitiesof drug need to be administered to attain a desired result. Althoughcareful clinical monitoring may prevent extensive damage or loss ofviable tissue, the providing of a pharmaceutical agent-carrier systemwhich is actively transported through standard biological systems to thetreatment site prior to activation of the pharmaceutical agent would behighly desirable not only in optimizing utilization of the drug but alsoin the reduction of side effects and/or the minimization of thedestruction of healthy cells. The direct injection of cytotoxic agentsinto solid tumors of the breast, bladder, prostate and lung usingconventional cytotoxic chemotherapeutic agents as adjuvants to surgeryand/or radiotherapy has been of limited success in prolonging the livesof patients. This is partially due to the dose limitations imposed bythe acute and chronic toxicity to tissues or organ systems beyond thosethat are targeted.

As it relates to the administration of cytotoxic or antineoplasticdrugs, the effective resolution of concerns relating to modes ofadministration, to the limitation of dosage size and frequency ofadministration, and to side effects would certainly be of benefit to thetreatment of cancer.

Oligonucleotides that specifically interfere with gene expression at thetranscriptional or translational levels have the potential to be used astherapeutic agents to control the synthesis of deleterious proteinsassociated with viral, neoplastic or other diseases. It is possible toselect single-stranded oligonucleotides that recognize and bind to themajor groove of a stretch of double-stranded DNA in a sequence-specificmanner to form a triple helix (Le Doan et al., 1987; Moser and Dervan,1987). Triple helix-forming oligonucleotides targeted to the promoterregion of certain genes have been used to physically block RNA synthesisin cell-free transcription assays (Cooney et al., 1988; Postel et al.,1992; Skoog et al., 1993; Rando et al., 1994). Similarly, in vitrotranslation assays have been used to demonstrate that antisenseoligonucleotides can bind mRNA targets and prevent protein synthesis(Uhlmann and Peyman, 1990; Cohen and Hogan, 1994).

Although antisense oligonucleotides have shown great efficacy in theselective inhibition of gene expression (Stein and Cohen, 1988; Szczyliket al., 1991; Gray et al., 1993), the therapeutic applications of suchantisense oligonucleotides are currently limited by their lowphysiological stability, slow cellular uptake, and lack of tissuespecificity. The instability obstacles have been largely overcome by useof backbone-modified oligonucleotides that are more resistant tonucleases. Methylphosphonates, protein-nucleic acid conjugates, andphosphorothioates all appear to resist enzymatic digestion better thanthe corresponding natural oligonucleotides (Chang and Miller, 1991;Wickstrom et al., 1992; Letsinger, 1993; Zon, 1993).

Problems with cellular uptake of antisense oligonucleotides have beenmore difficult to solve. Endogenous uptake pathways that rely onpinocytosis and related processes generally have insufficient capacityto deliver the quantities of antisense of oligonucleotides required tosuppress gene expression (Vlassov et al., 1994). Hydrophobicmodifications have also been undertaken to improve membranepermeability, but such derivatization strategies are most useful onlyfor short olgonucleotides (Vlassov et al., 1994). Although complexes ofantisense constructs with cationic liposomes or immunoliposomes (Gao andHuang, 1991; Bennett et al., 1992, Ma and Wei, 1996) and polylsine(Trubetskoy et al., 1992; Bunnell et al., 1992) have significantlyenhanced intracellular delivery, they have simultaneously introduced newdisadvantages of their own. Thus, both methods exhibit some carriercytotoxicity, and like other protocols, neither strategy allows for anytissue or cell targeting. In short, intracellular delivery and tissuespecificity remain major obstacles to the implementation of antisensedrugs in the treatment of human disorders.

Other techniques for the delivery of oligonucleotides to cells includethe use of: (a) folate-PEG-liposome constructs for the delivery ofantisense DNA against growth factor receptor (Wang et al., 1995); (b)folic acid-polylysine constructs for the delivery of c-myc antisense DNA(Ginobbi et al., (1997); (c) tris(N-acetylgalactosamine aminohexylglycoside) amide of tyrosyl(glutamyl)-glutamate (YEE(GaINAcAH)₃) linkedto polylysine for the delivery of DNA to cells via theasialoglycoprotein receptor (Merwin et al., 1994); and (d) water-solubleblock polycations (Kabanov et al., 1995).

It has been known for some time that a pharmaceutically active agent canbe attached to a carrier or molecule. The term “prodrug” is oftenassociated with such systems wherein the active agent is bonded toanother molecule for purposes of administration. The drug is usuallyinactive in the prodrug state and the bond is later cleaved releasingthe drug at a site where it can be effective. However, such systems arenot as useful as might be desired for various reasons, site specificitybeing one. Also, the release of the drug from its carrier requires thepresence of some agent or event to separate the active drug from itscarrier or molecule and, as such, may rely on factors such as thepresence of a specific enzyme, pH conditions, time release and the like,which may be variable from host to host and which may not be effectivelyimplemented.

For example, transmembrane transport of nutrient molecules is a criticalcellular function. Because practitioners have recognized the importanceof transmembrane transport to many areas of medical and biologicalscience, including drug therapy, peptide therapy and gene transfer,there have been significant research efforts directed to theunderstanding and application of such processes. Thus, for example,transmembrane delivery of nucleic acids has been encouraged through theuse of protein carriers, antibody carriers, liposomal delivery systems,electroporation, direct injection, cell fusion, vital carriers, osmoticshock, and calcium-phosphate mediated transformation. However, many ofthose techniques are limited both by the types of cells in whichtransmembrane transport is enabled and by the conditions of use forsuccessful transmembrane transport of exogenous molecular species.Further, many of these known techniques are limited in the type and sizeof exogenous molecule that can be transported across a membrane withoutloss of bioactivity.

One method for transmembrane delivery of exogenous molecules having awide applicability is based on the mechanism of receptor-mediatedendocytotic activity. Unlike many other methods, receptor-mediatedendocytotic activity can be used successfully both in vivo and in vitro.Receptor-mediated endocytosis involves the movement of ligands bound tomembrane receptors into the interior of an area bounded by the membranethrough invagination of the membrane. The process is initiated oractivated by the binding of a receptor-specific ligand to the receptor.Many receptor-mediated endocytotic systems have been characterized,including those recognizing galactose, mannose, mannose 6-phosphate,transferrin, asialoglycoprotein, transcobalamin (Vitamin B₁₂),α-2-macroglobulins, insulin, and other peptide growth factors such asepidermal growth factor (EGF).

Receptor-mediated endocytotic activity has been utilized for deliveringexogenous molecules such as proteins and nucleic acids to cells.Generally, a specified ligand is chemically conjugated by covalent,ionic or hydrogen bonding to an exogenous molecule of interest (i.e. theexogenous compound), forming a conjugate molecule having a moiety (theligand portion) that is still recognized in the conjugate by a targetreceptor. Using this technique, the phototoxic agent psoralen has beenconjugated to insulin and internalized by the insulin receptorendocytotic pathway (Gasparro, 1986); the hepatocyte-specific receptorfor galactose terminal asialoglycoproteins has been utilized for thehepatocyte-specific transmembrane delivery ofasialoorosomucoid-poly-L-lysine non-covalently complexed to a DNAplasmid (Wu, 1987); the cell receptor for epidermal growth factor hasbeen utilized to deliver polynucleotides covalently linked to EGF to thecell interior (Myers, 1988); the intestinally situated cellular receptorfor the organometallic Vitamin B₁₂-intrinsic factor complex has beenused to mediate delivery to the circulatory system of a vertebrate hosta drug, hormone, bioactive peptide or immunogen complexed with VitaminB₁₂ and delivered to the intestine through oral administration(Russell-Jones et al., 1995); the mannose-6-phosphate receptor has beenused to deliver low density lipoproteins to cells (Murray and Neville,1980); the cholera toxin binding subunit receptor has been used todeliver insulin to cells lacking insulin receptors (Roth and Maddox,1983); the human chorionic gonadotropin receptor has been employed todeliver a ricin a-chain coupled to HCG to cells with the appropriate HCGreceptor in order to kill the cells (Oeltmann and Heath, 1979); thetransferrin receptor has been used to deliver mitomycin C to sarcomacells (Tanaka et al., 1996) or to deliver doxorubicin tomultidrug-resistant cells (Fritzer et al., 1996);the biotin receptor hasbeen employed to deliver hypoxanthine-guanine phosphoribosyl transferase(HGPRT) by biotinylating the HGPRT to restore growth to HGPRT deficientcells (Low et al., 1995); and the folic acid receptor has been used todeliver antisense DNA to src-transformed fibroblast cells (Low et al.,1995).

Russell-Jones et al. (1995), describes a system which involves theformation of a covalent bond between the pharmaceutical agent one wishesto deliver and a modified Vitamin B₁₂ to form a conjugate molecule. Theconjugate is orally administered and is then transported from theintestinal lumen to the circulation. Importantly, the pharmaceuticalagent and the vitamin are bound through an amide linkage which is proneto acid hydrolysis. Russell-Jones et al. found that many biologicallyactive pharmaceutical agents can be bound to B₁₂ for facilitating theintroduction of the drug into the blood stream through oraladministration. Importantly, no method was provided whereby the drug-B₁₂bond could be selectively cleaved, nor could location of the activepharmaceutical agent be controlled once activated. Instead,Russell-Jones et al. relied on biochemical degradation of the drug-B₁₂bond to release the drug in its active form. Importantly, under thismethod the drug could be released in its active form anywhere within thecirculation system, diminishing the importance of the active transportof B₁₂ into cancer tissue. Moreover, the conjugates formed under thismethod require the modification of the structure of the corrin ring ofthe B₁₂ molecule, which modification can have serious effects onreceptor interactions.

Thus, there exists a need for a drug delivery system which can beutilized for the delivery of bioactive agents, includingpharmaceuticals, peptides and oligonucleotides. There is also a need fora drug delivery system which can be used for site-specific release ofthe bioactive agent in the cells, tissues, or organs in which atherapeutical effect is desired to be effected.

SUMMARY OF THE INVENTION

The present invention relates to bioconjugates and the delivery ofbioactive agents which are preferably targeted for site specific releasein cells, tissues or organs. More particularly, this invention relatesto bioconjugates which comprise a bioactive agent and an organocobaltcomplex. The bioactive agent is covalently bonded directly or indirectlyto the cobalt atom of the organocobalt complex. The bioactive agent isreleased from the bioconjugate by the cleavage of the covalent bondbetween the bioactive agent and the cobalt atom in the organocobaltcomplex, as described herein.

The bioactive agent is any agent which is desired to be delivered tocells, tissues or organs for nutrient or therapeutic effects. Inaccordance with the present invention, bioactive agents include, but arenot limited to, nutrients, pharmaceuticals, drugs, peptides andoligonucleotides.

The organocobalt complex is any organic complex containing a cobalt atomhaving bound thereto 4-5 nitrogen and/or chalcogens such as oxygen,sulfur, etc., as part of a multiple unsaturated heterocyclic ringsystem. In accordance with the present invention, suitable organocobaltcomplexes include, but are not limited to, cobalamin, Co[SALEN],organo-(pyridine)bis(dimethylglyoximato)cobalt, corrinoids, derivativesthereof and analogues thereof.

The organocobalt complexes may be unsubstituted or substituted withconventional organic functional groups which will not alter the basicnature of the organocobalt complex. The basic is nature of theorganocobalt complex is to directly or indirectly bind the bioactiveagent covalently to the cobalt such that the cobalt-bioactive agent bondis readily cleavable as described herein. The organocobalt complex mayalso be covalently bound directly or indirectly to a targeting molecule.The targeting molecule is a molecule for which the desired cell, tissueor organ has a requirement or a receptor, as described herein.

The bioconjugate according to the present invention is administered to asubject in need of therapeutic treatment. The bioconjugate concentratesin a targeted cell, tissue or organ site as a result of the organocobaltcomplex. As an example, a bioconjugate containing a chemotherapeutic isadministered to a patient and the bioconjugate concentrates inneoplastic cells where the active chemotherapeutic is released from thebioconjugate by cleavage. Similarly, other pharmaceuticals, drugs,peptides or oligonucleotides are administered to a subject as part ofthe bioconjugate which is concentrated in the desired cells, tissues ororgans. The pharmaceuticals, drugs, peptides or oligonucleotides arereleased by cleavage. In one embodiment, the cleavage may occur as aresult of normal displacement by cellular nucleophiles or enzymaticaction. In a second embodiment, the cleavage is caused to occurselectively at the release site by an external signal. The externalsignal may be light or photoexcitation, i.e. photolysis, or it may beultrasound, i.e. sonolysis. Further, if the photolysis takes place inthe presence of a magnetic field surrounding the release site therelease of the drug, such as a cytotoxic agent, into surrounding healthytissue can be minimized.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the structure and absorption spectrum of methylcobalamin(B₁₂).

FIG. 2 shows the structure and absorption spectrum of ethyl-Co[SALEN](cobalt-bis-[salicylidene]-ethylenediamine.

FIG. 3A shows a sequential absorption spectra of aqueous CH₃-Cbl^(III)as a function of anaerobic sonolysis (pH 7.38, 100 mM Hepes, saturatingAr).

FIG. 3B shows the change in absorbance spectra following aerobicsonolysis in the absence of organic buffer.

FIG. 4A shows a sequential absorption spectra of aqueous compound 3(Example 6) as a function of anaerobic sonolysis at pH 7.4, 100 mMHepes, saturating Ar.

FIG. 4B shows the change in absorbance spectra following aerobicsonolysis of a compound 3 (Example 6) solution containing phosphatebuffer.

FIG. 5 shows the effect of a chlorambucil bioconjugate on cell viabilityfor the HCT-116 cell line. The results are shown for chlorambucil (▪),the chlorambucil bioconjugate with photolysis (∘), the chlorambucilbioconjugate with no photolysis (▴) and the chlorambucil bioconjugateplus 10 equivalents of hydroxycobalamin with photolysis (∇).

FIG. 6 shows the effect of a chlorambucil bioconjugate on cell viabilityfor the HL-60 cell line. The results are shown for chlorambucil (▪), thechlorambucil bioconjugate with no photolysis (▴) and the chlorambucilbioconjugate plus 10 equivalents of hydroxycobalamin with no photolysis(∇).

FIG. 7 shows the effect of a chlorambucil bioconjugate on cell viabilityfor the B-16 cell line. The results are shown for chlorambucil (▪), thechlorambucil bioconjugate with photolysis (∘), the chlorambucilbioconjugate with no photolysis (▴) and the chlorambucil bioconjugateplus 10 equivalents of hydroxycobalamin with photolysis (∇).

FIG. 8 shows the effect of a chlorambucil bioconjugate on cell viabilityfor the Meth-A cell line. The results are shown for chlorambucil (▪),the chlorambucil bioconjugate with photolysis (∘), the chlorambucilbioconjugate with no photolysis (▴) and the chlorambucil bioconjugateplus 10 equivalents of hydroxycobalamin with photolysis (∇).

FIG. 9 shows the effect of a chlorambucil bioconjugate on cell viabilityfor the RD-995 cell line. The results are shown for chlorambucil (▪),the chlorambucil bioconjugate with photolysis (∘), the chlorambucilbioconjugate with no photolysis (▴) and the chlorambucil bioconjugateplus 10 equivalents of hydroxycobalamin with photolysis (∇).

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to bioconjugates and the delivery ofbioactive agents which are preferably targeted for site-specific releasein cells, tissues or organs. More particularly, this invention relatesto bioconjugates which comprise a bioactive agent and an organocobaltcomplex. The bioactive agent is covalently bonded directly or indirectlyto the cobalt atom of the organocobalt complex. The bioactive agent isreleased from the bioconjugate by the cleavage of the covalent bondbetween the bioactive agent and the cobalt atom in the organocobaltcomplex. The cleavage may occur as a result of normal displacement bycellular nucleophiles or enzymatic action, but is preferably caused tooccur selectively at a predetermined release site by application of anexternal signal. The external signal may be light or photoexcitation,i.e. photolysis, or it may be ultrasound, i.e. sonolysis. Further, ifthe photolysis takes place in the presence of a magnetic fieldsurrounding the release site, the release of the bioactive agent intosurrounding healthy tissue is minimized.

The bioconjugate according to the present invention is administered to asubject in need of therapeutic treatment. The bioconjugate concentratesin a targeted cell, tissue or organ site as a result of the organocobaltcomplex. The bioactive agent is released from the bioconjugate bycleavage. In one embodiment, the cleavage may occur as a result ofnormal displacement by cellular nucleophiles or enzymatic aciton. In asecond embodiment, the cleavage is caused to occur selectively at therelease site by an external signal. The external signal may be light orphotoexcitation, i.e. photolysis, or it may be ultrasound, i.e.sonolysis. Further, if the photolysis takes place in the presence of amagnetic field surrounding the release site, the release of the drug,such as a cytotoxic agent, into surrounding healthy tissue is minimized.

As one example, the bioconjugate contains a chemotherapeutic agent andis administered to a patient having cancer. In this example, atherapeutically effective amount of the bioconjugate is administeredintravenously to a patient such that the bioconjugate concentrates inthe neoplastic cells. The chemotherapeutic agent is released from thebioconjugate by natural means (e.g., cellular nucleophiles or enzymaticaction) or preferably by means of an external signal (e.g., light orultrasound).

As a second example, the bioconjugate contains a cytotoxic agent and isadministered to a patient having psoriasis. In this example, atherapeutically effective amount of the bioconjugate is administered toan afflicted skin site. The cytotoxic agent is released by natural meansor preferably by means of an external signal.

As a third example, the bioconjugate contains the enzymatic domain ofdiphtheria toxin (Nichols et al., 1997) and is administered to a patienthaving cancer. In this example, a therapeutically effective amount ofthe bioconjugate is administered intravenously to a patient such thatthe bioconjugate concentrates in the neoplastic cells. The enzymaticdomain of diphtheria toxin is released from the bioconjugate by naturalmeans (e.g., cellular nucleophiles or enzymatic action) or preferably bymeans of an external signal (e.g., light or ultrasound) and proceeds tokill the cancer cells.

As a fourth example, the bioconjugate contains an antisenseoligonucleotide against hepatitis B virus (Yao et al., 1996; Madon andBlum, 1996) and is administered to a subject having hepatitis B. In thisexample, a therapeutically effective amount of the bioconjugate isadministered intravenously to a patient such that the bioconjugateconcentrates in the liver. The antisense oligonucleotide is releasedfrom the bioconjugate by natural means (e.g., cellular nucleophiles orenzymatic action) or preferably by means of an external signal (e.g.,light or ultrasound) and proceeds to inhibit gene expression andreplication of hepatitis B virus.

The present invention employs the following definitions:

Bioactive agent: any agent which is desired to be delivered to cells,tissues or organs for modulating or otherwise modifying cell function,including for therapeutic effects. In accordance with the presentinvention, bioactive agents include, but are not limited to,pharmaceutically active compounds or diagnostic compounds. Bioactiveagents include, but are not limited to, peptides, oligopeptides,proteins, apoproteins, glycoproteins, antigens and antibodies orantibody fragments thereto, receptors anti other membrane proteins,retro-inverso oligopeptides, protein analogs in which at least onenon-peptide linkage replaces a peptide linkage, enzymes, coenzymes,enzyme inhibitors, amino acids and their derivatives, hormones, lipids,phospholipids, liposomes, ricin or ricin fragments; toxins such asaflatoxin, digoxin, xanthotoxin, rubratoxin; antibiotics such ascephalosporins, penicillin and erythromycin; analgesics such as aspirin,ibuprofen and acetaminophen, bronchodilators such as theophylline andalbuterol; beta-blockers such as propranolol, metoprolol, atenolol,labetolol, timolol, penbutolol and pindolol; antimicrobial agents suchas those described above and ciprofloxacin, cinoxacin and norfloxacin;antihypertensive agents such as clonidine, methyldopa, prazosin,verapamil, nifedipine, aptopril and enalapril; cardiovascular agentsincluding antiarrhythmics, cardiac glycosides, antianginals andvasodilators, central nervous system agents including stimulants,psychotropics, antimanics and depressants; antiviral agents;antihistamines such as chlorphenirmine and brompheniramine; cancer drugsincluding chemotherapeutic agents, such as chlorambucil, carboplatin,deratives of busulfan, doxorubicin, etoposide, topotecan (TPT);tranquilizers such as diazepam, chordiazepoxide, oxazepam, alprazolamand triazolam, anti-depressants such as fluoxetine, amitriptyline,nortriptyline and imipramine; H-2 antagonists such as nizatidine,cimetidine, famotidine and ranitidine, anticonvulsants; antinauseants;prostaglandins; muscle relaxants; anti-inflammatory substances;stimulants; decongestants; antiemetics; diuretics; antispasmodics;antiasthmatiics; anti-Parkinson agents; expectorants; coughsuppressants, mucolytics; vitamins; and mineral and nutritionaladditives. Other molecules include nucleotides; oligonucleotides;polynucleotides; and their art-recognized and biologically functionalanalogs and derivatives including, for example, methylatedpolynucleotides and nucleotide analogs having phosphorothioate linkages;plasmids, cosmids, artificial chromosomes, other nucleic acid vectors;antisense polynucleotides including those substantially complementary toat least one endogenous nucleic acid or those having sequences with asense opposed to at least portions of selected viral or retroviralgenomes; promoters; enhancers; inhibitors; other ligands for regulatinggene transcription and translation. In addition, the bioactive agent canbe any other biologically active molecule that can form a conjugate withan organocobalt complex. The bioactive agent may further contain aspacer which provides a covalent bond with the cobalt atom of theorganocobalt complex, but which does not adversely affect the biologicalactivity of the bioactive agent.

Bioconjugate: a conjugate containing a bioactive agent and anorganocobalt complex in which the bioactive agent is covalently bounddirectly to the cobalt atom or is covalenly bound indirectly to thecobalt atom via a spacer.

Non-reactive atom: an atom in the bioactive agent that will not lead torearrangement or destruction of the bioactive agent under conditions ofligand exchange during receptor-mediated endocytosis, but that insteadwill reproduce the original form of the bioactive agent (or bioactiveagent and spacer) and thereby unmask an active bioactive agent. Thenon-reactive atom may be a carbon atom, a nitrogen atom, an oxygen atom,a sulfur atom, a selenium atom or a silicon atom. A carbon atom (e.g.from an alkyl, acyl or aryl group) is particularly preferred. Suchnon-reactive atoms are also used in forming the covalent bond betweenthe cobalt and the spacer.

Organocobalt complex: an organic complex containing a cobalt atom havingbound thereto 4-5 calcogens as part of a multiple unsaturatedheterocyclic ring system. In accordance with the present invention,suitable organocobalt complexes include, but are not limited to,cobalamin (coenzyme B₁₂), Co[SALEN] (which is a cobalamin analogue),organo(pyridine)-bis(dimethylglyoximato)cobalt, corrinoids (such asdisclosed by Brown et al., 1996) and derivatives or analogues of any ofthe preceding, as well as pharmaceutically acceptable salts. Theorganocobalt complexes may be unsubstituted or substituted withconventional organic functional groups which will not alter the basicnature of the organocobalt complex. The basic nature of the organocobaltcomplex is to bind the bioactive agent covalently to the cobalt suchthat the cobalt-bioactive agent bond is readily cleavable as describedherein. Examples of substituents which may be found on the organocobaltcomplex include amino, nitro, halogen (bromine, chlorine), sulfito,C₂₋₆-alkene and C₂₋₆ alkyne. For example, the organocobalt complex canbe formed having a nitro and/or halo (e.g., bromo) derivative of thecorrin ring or having an extended conjugation with exocyclic olefin oralkylene groups. Other derivatives include cobalamin lactone, cobalaminlactame and those in which the benzimidiazole ring (e.g., of cobalamin,green corrinoid, and the like) are substituted with e.g., one or morehalogen (bromine, chlorine), hydroxy or C₁₋₆ alkyl. Such substituentsare useful for increasing the λ_(max) to be used for cleavage of thebioconjugate as described herein. Further derivatives include anilide,ethylamide, mono-, di- or tri-carboxylic acid or proprionamidederivatives of cobalamin of Vitamin B₁₂. In one embodiment, theorganocobalt complex is any organic complex conaining cobalt which isbound by transcobalamin and transported into a cell by areceptor-mediated process involving transcobalamin. In a secondembodiment, the organocobalt complex may also be covalently bounddirectly or indirectly (through a spacer) to a targeting molecule,wherein said targeting molecule is bound by its receptor and the complexis transported into a cell by a receptor-mediated process. Co[SALEN] andits derivatives or analogues can be represented by the general formula

wherein the substitutents may be included or omitted to modulatephysical properties of the molecule, e.g., water solubility, stabilityor λ_(max)—the wavelength at which the complex absorbs. Thus, thesubstituents are as follows: R is H, a group which increases watersolubility and/or stability or a group for attachment of a targetingmolecule and W, W′, X, X′, Y, Y′, Z and Z′ are independently H, a groupwhich increases water solubility and/or stability, a group forattachment of a targeting molecule or a group for modified absorbance ofenergy, or W and X together and W′ and X′ together are a 4-6 membercyclic or heterocyclic ring, or Y and Z together and Y′ and Z′ togetherare a 4-6 member cyclic or heterocyclic aromatic ring. Examples ofgroups for enhancing water solubility include amino, C₁₋₆ alcohol, C₁₋₆carboxyl for any substitutent, or also SO₃— for the substitutents otherthan R. Examples of groups for attachment of a targeting moleculeinclude amino, C₁₋₆ alcohol and C₁₋₆ carboxyl for any substitutent.Examples of groups for modifying absorbance include CH₂OH, CO₂H, SO₃—,amino and nitro for the substitutents other than R. Such groups areuseful for increasing the wavelength of light to be used for cleavage ofthe bioconjugate as described herein, while targeting molecules areuseful in selectively targeting the bioconjugate to the desired tissue.Therefore, when used in the context of the present application, the termorganocobalt complex, unless specifically identified, shall be inclusiveof B₁₂ in all its embodiments, including coenzyme B₁₂, Co[SALEN] andother B₁₂ or B₁₂-like molecules, the organocobalt complexes definedherein, as well as any derivatives and analogues thereof.

Spacer: an atom or molecule which covalently binds together twocomponents. In the present invention, a spacer is intended to includeatoms and molecules which can be used to covaltently bind a bioactiveagent to the cobalt atom of an organocobalt complex or to covalentlybind a targeting molecule to an organocobalt complex. The spacer mustnot prevent the binding of the organocobalt complex or the targetingmolecule with its appropriate receptor. Examples of suitable spacersinclude, but are not limited to, polymethylene [—(CH₂)_(n), where n is1-10], ester [bioactive agent attached to O and Co to C═O], carbonate,ether, acetal or any combination of two or more of these units. Askilled artisan will readily recognize other spacers which can be usedin accordance with the present invention.

Several of these spacers are useful as a “self-destructing” linkergroup. That is, some or all of the linkage would be consumed in afragmentation reaction. This means that, following cleavage of the C—Cobond by photolysis or sonolysis, an additional cleavage will take placeseveral bonds away, leading to the formation of a small, unsaturated(and typically volatile) molecule made up of atoms of the former linker.This is shown schematically below:

The most typical scenario is the subsequent cleavage of a second bond,two bonds removed from the first. Thus, most self-destructive linkerswould contain a two-atom unit whose extrusion as a small, gaseousmolecule is favorable. Another design feature is to have the new radicalspecies which is generated after the second cleavage step be anespecially stable kind of radical. Examples of self-destructing linkersare shown below:

Targeting Molecule: a molecule which is bound by a receptor andtransported into a cell by a receptor-mediated process. Examples ofsuitable targeting molecules include, but are not limited to, glucose,galactose, mannose, mannose 6-phosphate, transferrin,asialoglycoprotein, α-2-macroglobulins, insulin, a peptide growthfactor, cobalamin, folic acid or derivatives, biotin or derivatives,YEE(GalNAcAH)₃ or derivatives, albumin, texaphyrin, metallotexaphyrin,porphyrin, any vitamin, any coenzyme, an antibody, an antibody fragment(e.g., Fab) and a single chain antibody variable region (scFv). Askilled artisan will readily recognize other targeting molecules(ligands) which bind to cell receptors and which are transported into acell by a receptor-mediated process. The present invention is intendedto include all such targeting molecules.

The present invention takes advantage of the cellular properties ofcobalamin and cobalamin analogues or derivatives, as well as thecellular properties of other targeting molecules. For example, studieshave shown that the absorption of physiological amounts of vitamin B₁₂by the gut requires that it be complexed with a naturally occurringtransport protein known as intrinsic factor (IF). (Castle, 1953; Fox andCastle, Allen and Majerus, 1972b). This protein is released into thelumen of the stomach by parietal cells in the fundus. Once bound tointrinsic factor, the B₁₂-IF complex interacts with a membrane boundreceptor for IF located on the terminal ileum of the small intestine.The receptor-IF-B₁₂ complex is then internalized by a process ofreceptor-mediated endocytosis (RME). Allen and Majerus demonstrated thatit is possible to chemically modify B₁₂, couple it to a resin and usethe B₁₂-resin to affinity purify IF (Allen and Majerus, 1972a). Thisfinding suggests the possibility of coupling a large macromolecule (suchas the resin used by Allen and Majerus, 1972a) to B₁₂ while stillpreserving its ability to interact specifically with intrinsic factorand thus be part of the active transport system. By coupling moleculesto B₁₂ in such a way as to preserve the ability of B₁₂ to interact withintrinsic factor, it was found that the natural uptake mechanism fororally administered B₁₂ could be used to deliver various proteins, drugsor other pharmaceutically active molecules from the intestinal lumen tothe circulation. It has been found that B₁₂ is naturally concentrated incancer tissue through a similar transport mechanism.

In mammals, B₁₂ is transported in the blood by transcobalamin proteinsTC-I, TC-II, and TC-III. The major form of B₁₂ in the blood ismethylcobalamin and the largest store of B₁₂ is adenosylcobalamin in theliver. Rapidly dividing cells, including cancer cells, require coenzymeB₁₂ for thymidine production during DNA synthesis. It has been reportedby Carmel (1975) that, in some patients with tumors, up to 50-foldincreases in the major cobalamin transport proteins TC-I and TC-II havebeen observed. Waxman et al. (1972), report the finding of tumorspecific B₁₂ binding proteins that circulate in the blood. In eachinstance, these increases in TC transport proteins and the correspondingsystemic aepletion of B₁₂ were not the result of megaloblastosis,granulocyte proliferation, or any other pathogenic B₁₂ deficiency.

In a second example of receptor-mediated endocytosis, folate receptorsthat mediate endocytotic activity have previously been identified inbacterial cells (Kumar et al., 1987) and used for delivery ofbiologically active materials (Low et al., 1995). Folic acid, folinicacid, pteropolyglutamic acid, and folate receptor-binding pteridinessuch as tetrahydropterins, dihydrofolates, tetrahydrofolates and theirdeaza and dideaza analogs are useful as targeting molecules inaccordance with the present invention. The terms “deaza” and “dideaza”analogs refer to the art-recognized analogs having a carbon atomsubstituted for one or two nitrogen atoms in the naturally-occurringfolic acid structure. For example, the deaza analogs include the1-deaza, 3-deaza, 5-deaza, 8-deaza, and 10-deaza analogs. The dideazaanalogs include, for example, 1,5-dideaza, 5,10-dideaza, 8,10-dideaza,and 5,8-dideaza analogs. The foregoing folic acid deriatives areconventionally termed “folates,” reflecting their capacity to bind withfolate-receptors, and such ligands when complexed with exogenousmolecules are effective to enhance trans-membrane transport. Otherfolates useful as complex forming ligands for this invention are thefolate receptor binding analogs aminopterin, amethopterin(methotrexate), N-methylfolate, 2-deamino-hydroxyfolate, deaza analogssuch as 1-deazamethopterin or 3-deazamethopterin, and3′5′-dichloro-4-amino-4-deoxy-N¹⁰-methylpteroyl-glutamic acid(dichloromethotrexate). Other suitable ligands capable of binding tofolate receptors to initiate receptor-mediated endocytotic transport ofthe complex include anti-idiotypic antibodies to the folate receptor. Anexogenous molecule in complex with an anti-idiotypic antibody to afolate receptor is used to trigger trans-membrane transport of thecomplex. Such molecules are used in accordance with the presentinvention as a targeting molecule.

In a further example of receptor-mediated endocytosis, biotin receptorshave been used to mediate endocytotic activity (Low et al., 1995).Biotin analogs such as biocytin, biotin sulfoxide, oxybiotin and otherbiotin receptor-binding compounds are ligands that may also be used assuitable targeting molecules to promote the trans-membrane transport ofexogenous molecules in accordance with this invention. Other compoundscapable of binding to biotin receptors to initiate receptor-mediatedendocytotic transport of the complex are also contemplated. These caninclude other receptor-binding ligands such as, for exmple,anti-idiotypic antibodies to the biotin receptor. An exogenous moleculecomplexed with an anti-idiotypic antibody to a biotin receptor could beused to trigger trans-membrane transport of the complex. Such moleculesare used in accordance with the present invention as a targetingmolecule.

Other examples of targeting molecules include glucose, galactose,mannose, mannose 6-phosphate, hormones (e.g., insulin, growth hormone,and the like), growth factors or cyokines (e.g., TGF-β, EGF,insulin-like growth factor, and the like), YEE(GalNAcAH)₃ orderivatives, cobalamin, α-2 macroglobulins, asialoglycoprotein, albumin,texaphyrin, metallotexaphyrin, antibodies, antibody fragments (e.g.,Fab), single-chain antibody variable region (scFv), transferrin, anyvitamin and any coenzyme.

As previously described, a bioconjugate of the present inventioncomprises a bioactive agent conjugated directly or indirectly via acovalent bond to the cobalt atom of an organocobalt complex. Thebioactive agent is conjugated directly to the cobalt atom through anon-reactive atom in the bioactive agent or is conjugated indirectly tothe cobalt atom through the use of a spacer. Therefore, in contrast tothe conjugates formed under U.S. Pat. No. 5,428,023, the attachment of abioactive agent to the cobalt atom in the axial position does notinterfere with receptor-mediated endocytosis from the blood into cells.

The unusually weak cobalt-non-reactive atom bond (e.g., C—Co bond) ofthe bioconjugate provides a readily addressable trigger for thecontrolled in vivo release of the bioactive agent from the organocobaltcomplex. The bond dissociation energy (BDE) of Co-non-reactive atom bondin the bioconjugate is in the range of 30 to 50 kcal/mol (e.g., 30-40kcal/mol range for a Co—C bond) which make them among the weakestcovalent bonds known, yet the bond is relatively stable in aqueoussolution.

A common strategy will employ the modification of the anticancer drug sothat it possesses an electrophilic site which can react with the highlynucleophilic Co(I) intermediate generated upon treatment ofhydroxycobalamin with NaBH₄. This structural modification will besufficiently far removed from the active site (pharmacophore) topreclude any interference with the desired biological activity.Approache used in the case of chlorambucil are typical: the carboxylicacid group of chlorambucil is converted to either an acid chloride or abromoethyl ester, either of which can be efficiently coupled withcob(I)alamin.

For example, reduced Cbl^(I) is prepared by NaBH₄ or zinc dustreduction, e.g. of hydroxocob(III)alamin. In the above scheme, the drugcan be a cytotoxic agent, other drug or other bioactive agent asdescribed herein. In other schemes, a spacer containing a carbon atom orother atom such as that specified for the non-reactive atom for bindingto the cobalt atom and which also contains a reactive grouping, e.g. —OHor —CN, which is further reacted with the bioactive agent, isintroduced. Other reactive groups, e.g. —NH₂, —SH, —COOH, etc., can alsobe utilized for coupling to a bioactive agent. It is important to notethat, in some cases (e.g., chlorambucil, doxorubicin), the small organicmolecule released is not the parent drug, but rather retains some of themodification installed to allow coupling. In other cases (e.g.,topotecan), the structure of the released drug may correspond to theparent molecule.

More specific details of the synthesis of representative bioconjugatesaccording to the present invention are as follows, using a “drug” whichcan be replaced by any suitable bioactive agent and cobalamin which canbe replaced by any suitable organocobalt complex. In this synthesis, allprocedures are under argon. Hydroxocob(III)alamin is dissolved inaqueous CH₃OH (1:1 v/v) at 25° C. A 2-10 fold excess of NaBH₄ is added.The solution slowly changes color from red to brown and gradually green(Cbll). After approximately 15 min. the electrophilic drug ligand(dissolved in the same deoxygenated solvent) is added, e.g., as analkyl, acyl or aryl chloride. Strictly anaerobic conditions aremaintained and the reaction mixture is stirred gently at 25° C. Thecolor gradually changes back to red as Cb^(I) is converted to alkyl-,acyl-, or aryl-Cbl^(III). After about 1.5 h, the solution is acidifiedto pH 3.0 with dilute HCl. Methanol is removed under reduced pressure byrotary evaporation at less than 40° C. The resulting aqueous solution isdiluted with an equal volume of H₂O and loaded onto a Dowex AG-50-X2(200-400 mesh) cation exchange column. The column is washed sequentiallywith H₂O and 0.1 M NaOAc, pH 6.4. Fractions containingdrug-cob(III)alamins appear red and are collected appropriately.Unreacted hydroxocob(III)alamin is retained on the column. Combinedfractions of drug-cob(III)alamin are extracted with phenol andconcentrated by rotary evaporation. Drug-cob(III)alamins can often becrystallized by the addition of acetone to a concentrated aqueoussolution. Characterization of the alkyl-, acyl-, or aryl-cobalaminconjugates is by NMR, mass spectrometry (FAB, Cl, or electrospray), andIR methods.

A methotrexate-containing bioconjugate can be synthesized by thefollowing methods.

In method one utilizing the above procedure, methotrexate (MTX) isconverted to its corresponding acyl chloride and reacted with cobalaminand/or Co(III)[SALEN] and/or other disclosed organocobalt complexes toyield methotrexate-cobalamin and methotrexate-Co(III)[SALEN] accordingto the following reaction scheme I. In the alternative method two, theC—Co bond is first formed from an acyl chloride having a protected aminogroup. The amino group is then deprotected, followed by formation of theamide bond to an aminobenzoylpterin according to the following reactionscheme II.

An aminopterin-containing bioconjugate can be synthesized by thefollowing method. The des-methyl derivative of methotrexate(aminopterin) is coupled to cobalt as shown by the following reactionformula, in which the iminium ion is either reacted with Co(I) directly,or the iminium ion is converted to the aminonitrile and then slowlyunmasked to reveal the iminium cation.

A topotecan-containing bioconjugate can be synthesized by the followingmethod. The cytotoxic activity of topotecan (TPT) or camptothecin (CPT)arises from their ability to freeze topoisomerase I-DNA “cleavablecomplexes.” (Pommier et al., 1995) Since some tumor types displaygreatly elevated levels of topo I (Giovanella et al., 1989),topoisomerase poisons of this type are likely to have a highertherapeutic index in the treatment of those cancers. However, treatmentwith camptothecin derivatives could be made more general if used inconjunction with the targeted delivery approach.

Topotecan is conjugated to cobalamin, Co[SALEN] and other organocobaltcomplexes according to the following reaction schemes. Camptothecin isconjugated in a similar manner. Preparation of 10a and 10b involvessimilar chemistry to that discussed above for 8a,b. Selective generationof the phenyl chloroformate (25) of topotecan (5) and acylation of Co(I)gives 10a. Exposure of 25 to 18 or treatment of 5 with the previouslydiscussed chloroformate 19 furnishes 10b. Conjugates 10c,d will requiresomewhat longer routes, as they cannot be prepared directly from 5.However, the established synthetic route for conversion of the naturalproduct camptothecin to 5 can be modified at the appropriate point toallow for attachment of the cobalt complex. The first three steps toprepare phenolic intermediate 26 are known (Mulliez et al., 1994).Mannich-type substitution with formaldehyde/dimethylamine then gives 5.Use of methylamine gives the corresponding secondary amine 27. At thispoint, linkage to Co via a methylene to give 10c is possible via Co(I)trapping of a second, in situ generated imminum salt. Alternatively,N-alkylation with 23 gives 10d. Cleavage of 10a and 10b provides 5directly via fragmentative pathway or indirectly via other products.Cleavage of 10c with hydrogen extraction yields 5. Cleavage of 10dyields the product 5 having an ethylmethylamino group in place of thedimethylamino group.

A busulfan-containing bioconjugate can be synthesized by the followingmethod. Busulfan is an alkylating agent used therapeutically againstchronic myelogenous leukemia (CML). The preferred point for attachingbusulfan to the organocobalt complex is on one of the alkanesulfonateunits. A slight change in the structure of the sulfonate portion of theester is will not exert a large effect on the ability of the releaseddrug to crosslink DNA. Cleavage of 7a followed by hydrogen abstractionfurnishes the mixed ethanesulfonate/methanesulfonate 2b. Trapping of thecarbon radical under oxidative conditions produces mixed bis(sulfonate)2c, which is also a competent crosslinking agent. Cleavage of 7b resultsin the release of the parent drug 2a after hydrogen abstraction.

Bis-methylsulfonate busulfan is conjugated to cobalamin, Co[SALEN] andother organocobalt complexes according to the following reactionschemes. For the preparation of 7a, the commercially available sodiumsalt of bromoethanesulfonic acid (11) serves as the starting point.Heating with phosphorus pentachloride furnishes the correspondingsulfonyl chloride 12 as a distillable liquid. Treatment with Co(I) leadsto preferential displacement of the bromide to furnish 13, which isconverted to 7a by sequential treatment with 1,4-butanediol and mesylchloride. The order of the final three steps can be changed; forexample, treatment of 12 with excess butanediol, followed by mesylchloride gives the mixed bis(sulfonate) 14. Selective displacement ofthe primary bromide by Co(I) then gives 7a. In the case of conjugate 7b,treatment of 2-bromobutane-1,4-diol (which is readily available frommalic acid diester) with Co(I) gives adduct 15. Bis(mesylation) gives7b. Alternatively, 7b is prepared from 16 (X═Br or I) with selectivedisplacement of the halide.

A chlorambucil-containing bioconjugate can be synthesized by thefollowing methods. Chlorambucil is a relatively stable nitrogen mustardwith attenuated alkylating ability, presumably as a consequence of theless-basic aniline nitrogen.

Method One: In this procedure, chlorambucil is converted to the acidchloride followed by reaction with cob(I)alainin or Co(I)[SALEN]according to reaction sequence I. In situations where the acyl linkageto the organocobalt complex is too labile towards serum nucleophiles,two alternate bioconjugation procedures can be utilized.

Method Two: The procedure involves bromination of a carbon atom adjacentto the carboxyl group under standard Hell-Vollhardt-Zelinski conditionsto permit attachment of the Co complex in the α-position according toreaction sequence II. In scheme II, replacement of the C—Co with C—Hprovides chlorambucil. The reactant stoichiometry, temperature, anddilutions conditions can be manipulated to avoid competing displacementof one of the chloroethyl groups, or of the C1 by S_(N)2 attack.

Method Three: The BOC-protected p-aminophenylacetaldehyde can beconjugated to the Co moiety, followed by formation of the activenitrogen mustard product according to the following reaction sequenceIII.

A chlorambucil, ethyl ester-containing bioconjugate can be synthesizedby the following methods. When conjugating a drug via a carboxyl group,as in the case of chlorambucil, linking the drug to the cobalamin via ahydroxyethyl tether may be desirable. This can be accomplished by one oftwo convenient routes, both of which are schematically illustratedbelow. First, 2-hydroxyethyl-cob(III)alamin can be readily prepared fromcob(I)alamin and bromoethanol. Esterification is carried out understandard conditions, i.e. by reaction of a carboxylic acid(chlorambucil) with an alcohol (2-hydroxyethylcob(III)alamin) in thepresence of dicyclohexylcarbodiimide (DCC) (or water-soluble derivativessuch as EDCI) and a catalytic amount of 4-N,N-dimethylaminopyridine(DMAP) and its hydrochloride salt (DMAP-HCl) in dichloromethane ortoluene. Alternatively, the ester-linked conjugate can be prepared byfirst forming the 2-bromoethyl ester of chlorambucil and then reactingthe ester with cob(I)alamin to provide the same product. The reactionschemes (I, II) are shown below. With this mode of attachment, cleavagefrom the bioconjugate leads to release of the ethyl ester ofchlorambucil according to reaction scheme III.

An etoposide-containing bioconjugate can be synthesized by the followingmethod. Etoposide is a semisynthetic derivative of the natural productepipodophyllotoxin that is widely used against a variety of tumors,especially small cell lung carcinoma and germ cell tumors (De Jong etal., 1995). It has also shown considerable promise in the treatment ofrefractory cases of ovarian and breast cancer. Etoposide appears tofunction as a topoisomerase II poison.

Etoposide is conjugated to cobalamin, Co[SALEN] and other organocobaltcomplexes according to the following reaction schemes. Bioconjugates 8aand 8b require conversion of the free phenol of etoposide (3) to thecorresponding chloroformate 17. Direct acylation with Co(I) givesacylCo(III) derivative 8a, while treatment with the previously describedhydroxyethylCo(III) derivative 18 furnishes carbonate 8b. Thisderivative is also available via acylation of 3 with the chloroformate19 derived from 18. Preparation of acetal-modified conjugate 8c may bemore challenging. The ethylene acetal of 3 can be hydrolyzed and thenthe acetal reformed using aldehyde 20a or dimethyl acetal 20b(Keller-Jusi et al., 1971). Compound 20a may also be accessed viacareful, selective oxidation of 18, while 20b should be available viaalkylation of the Co(I) derivative with conunerically availablebromoacetaldehyde dimethyl acetal. In addition, the acetal of glucosecan be formed and then the secondary alcohol of 21 can be glucosylated.Cleavage of 8a or 8b either give 3 directly via fragmentative pathways,or furnish products which can undergo eventual hydrolysis to 3. Trappingwith H. following homolysis of 8c would then furnish 3.

A doxorubicin-containing bioconjugate can be Synthesized by thefollowing method. Doxorubicin (4) is the most widely used of theanthracycline antibiotics, and is clinically useful against a broadspectrum of solid and hematological tumors. Like etoposide, doxorubicinappears to target topoisomerase II, ultimately leading to growth arrestand nonapoptotic cell death (Fornari et al., 1996; Ling et al., 1996).The clinical usefulness of doxorubicin is limited by nonspecifictoxicity, especially cardiotoxicity. Thus, it would appear to be aparticularly good candidate for selective delivery. This is confirmed byits frequent use in liposome-based methods (Hu et al., 1996; Longman etal., 1995; Hosada et al., 1995), as part of immunoconjugates (Johnson etal., 1995; Sivam et al., 1995), or in prodrug approaches (Svensson etal., 1995).

Doxorubicin conjugated to cobalamin, Co[SALEN] and other organocobaltcomplexes according to the following reaction schemes. For the synthesisbioconjugate 9a, the condensation of the daunosamine amino group withacyl-Co(III) complex 22 is performed. This reaction forms the2-pyrroline ring in analogy to published routes using4-iodobutyraldehyde and 5-iodo-2-pentanone.(9b) The acyclic tertiaryamine derivative 9b is available from 4 via initial reductive aminationwith acetaldehyde, then alkylation of the resulting secondary amine withthe mesylate 23 derived from 18. Alternatively, treatment of 4 withchloroformate 19 provides carbamate 9e. If alternative points ofattachment are desired, hydrazone-linked derivatives such as 9d can beused using simple cobalamin alkyl hydrazides such as 24, obtainable from23. The cleavage of these bioconjugates is shown in the reaction schemebelow.

A carboplatin containing bioconjugate can be synthesized as shown in thefollowing reaction scheme.

A peptide-containing bioconjugate can be synthesized by the followingmethods: (1) The C-terminal carboxyl group of the peptide can beactivated and used to acylate Co(I), in analogy to the acylation of Cowith chlorambucil acid chloride. (2) The C-terminal carboxyl group canbe esterified with bromoethanol, in analogy to the other chlorambucilroute, and the bromide displaced with Co(I). (3) The N-terminal aminogroup of the peptide can be treated with CH₂═O and Co(I) to form aCo(III)—CH₂—NH-peptide linkage, in analogy to the synthesis of thetopotecan bioconjugate. (4) A Co(III)-amino acid complex can beprepared, and used in a coupling step with the remainder of the peptide.These methods can involve attachment of the Co at either the N- orC-terninus, or via a side-chain. A longer linker may be employed in anyof these routes, if it is desirable to keep the cobalt complex furtherremoved from the peptide chain.

An oligonulceotide or nucleic acid containing bioconjugate can besynthesized by the following methods. In both methods, a phosphate esteris used to link the end of the nucleotide and a hydroxyethyl-Co group.This linkage can be accomplished by either directly couplingCo—CH₂CH₂—OH and Nucl-OPO₃ ²⁻, or by esterifying Nucl—OPO₃ ²⁻ withBr—CH₂CH₂—OH, then displacing the Br with Co(I), as above.

An unsymmetrically substituted Co[SALEN] complex can be prepared from5-amino-salicylaldehyde and the glycolate ether of2,5-dihydroxybenzaldehyde. The amino group, which is prepared fromcommercially available 5-nitrosalicylaldehyde, functions as theattachment for the targeting molecule (binding domain, BD) by way ofEDCI-catalyzed amide formation. The other molecule has a carboxylic acidunit attached for solubility enhancement. Coupling of these twomolecules with ethylenediamine and Co(II) acetate furnishes a mixture ofthree comlexes: the two symmetrical complexes and the mixed one. All ofthese are useful, although the one lacking a BD-unit attached to eitherside of th e SALEN is less preferred.

With regard to binding domains, two possiblities are shown: a cobalaminderivative, and a peptide. In the former case, the known carboxylic acidi s u sed to attach cobalamin to the amino group of the SALEN. Thisbioconjugate still usees cobalamin-based receptor-mediated endocytosisto get into the cell, but the drug is attached through the SALEN insteadof the cobalamin. The latter case uses a peptide known to bind to cellsurface receptors of tumor cells (e.g., a fragment of epidermal growthfactor), with the carboxyl terminus attached to the amino group on theSALEN. Alternatively, one of the glutamate carboxyl groups of folate isused to obtain a folate-based bioconjugate. In addition to connectingthe binding domain via an amide linkage, one could use reductiveamination if the targeting molecule contained an aldehyde(BD-CHO+SALEN-NH₂+NaBH₄), or one could use the carboxyl group on theother piece to form an amide or ester linkage. Many other approaches(e.g., ether formation, olefination by Wittig reaction, attachment via adiester or diamide linker, etc.) are also possible.

Extended benzenoid systems of the SALEN ligands are shown below.

As a starting point in their synthesis, any of the commerciallyavailable naphthalene diols can be used. The diols undergo formylationreactions to Furnish the molecules shown below. These moleucles are thenbe coupled with Co(II) acetate and various diamines to give the extendedCo[SALEN] complexes. The OH groups on the second ring can be leftintact, used to attach the binding domain, or modified to enhance watersolubility through attachment of a polar group, such as polyamine,polycarboxylic acid, or carbohydrate moiety.

Modification of quinolines and isoquinolines are also carried out togive pyridine-fused SALENs.

Along similar lines, SALENs derived from monocyclic heteroyclichydroxyaldehydes are made, examples of which are shown below.

A bioconjugate containing the “green corrinoid” (Brown et al., 1996) canbe synthesized as follows. The “green corrinoid” can be reduced inanalogy to cobalamin, and that the reduced corrinoid will react withiodomethane to form the methylCo(III) corrinoid. Since the methylCo(III)corrinoid exhibits similar behavior to natural cobalamin, similarconjugation procedures with the electrophilic drug derivatives describedabove can be carried out.

The bioconjugates of the present invention have the improved property ofbeing capable of targeted, selective release of the bioactive agent fromthe bioconjugate. The bioactive agent is is released from thebioconjugate by cleavage. In one embodiment, the cleavage may occur as aresult of normal displacement by cellular nucleophiles or enzymaticaciton. In a second embodiment, the cleavage is caused to occurselectively at the release site by an external signal. The externalsignal may be light or photoexcitation, i.e. photolysis, or it may beultrasound, i.e. sonolysis. Further, if the photolysis takes place inthe presence of a magnetic field surrounding the release site, therelease of the drug, such as a cytotoxic agent, into surrounding healthytissue can be minimized.

Although it is desired to cause the bioactive agent to be released atthe desired cells, tissue or organs, e.g., at the site of the tumor orother cancer cells, it is also desirable to protect adjacent tissuesfrom the negative side effects of such potent agents. The bioactiveagent is released from the bioconjugate at the targeted site preferablyby application of an external signal, such as light or ultrasound. Thephotolysis of the bioconjugates of the present invention occurs thoughcleavage of the Co—C bond to produce a solvent-caged radical pairconsisting of Co(II) and the bioactive agent radical (R.). Lott et al.(1995) demonstrate that alkylcob(III)alamin photolysis can undergomagnetic field dependent recombination. A magnetic field application of100-3000 gauss can be used to enhance radical pair recombination insurrounding tissue where drug release from the conjugate is not desired,leading up to at least a 2-fold decrease in photochemically-triggereddrug release in such surrounding healthy tissue.

The sonolysis of the bioconjugates of the present invention occursthrough cleavage of the Co-non-reactive atom bond in aqueous solution toproduce the bioactive agent and a Co(II) (e.g., cob(II)alamin(Cbl^(II))) under anaerobic conditions or the bioactive agent andaquoCo-(III) (e.g., aquocob(III)alamin (H₂O—Cbl^(III))) under aerobicconditions. In either event, sonolysis from the focused application ofultrasound, results in Co-non-reactive atom bond cleavage and theirreversible release of the bioactive agent from the organocobaltcomplex.

The bioconjugates of the present invention can undergo natural cleavageas follows. Bioconjugates may be cleaved by natural means such as byserum nucleophiles. Once inside the cell, cobalamin-drug bioconjugates(utilized here only as an example and not intended to limit theinvention) can undergo cleavage by a variety of mechanisms. Standard B₁₂ligand exchange mechanisms permit the displacement of the drug.Alternatively, cellular nucleophiles can attack at either the carbon orthe cobalt atoms of the Co—C bond. Cyanide is the most common example ofa nucleophile which attacks at cobalt, leading to cyanocob[III]alaminand a free drug in which the former C—Co bond has been replaced with aC—H bond. Thiols (such as are found in cysteine or glutathione) canattack at carbon, leading to a reduced cob[I]alamin and a free drugwhich incorporates the former thiol group. (e.g.,R—Co[III]+R′—SH+base→R—S—R′+Co[I]+base-H.) Hydroxide and other basicagents can also cleave organic ligands from Co[III] complexes, althoughthis typically occurs via an elimination process which alters thestructure of the ligand through incorporation of a new double bond. Inaddition, B₁₂ metabolic enzymes present in cells can result in thecleavage of the bioactive agent from the co-atom.

The bioconjugates of the present invention can undergo cleavage byphotoactivation or photolysis as follows. The photochemical release ofthe bioactive agent from the organocobalt complex can be triggered bythe application of visible light at 400-800 nm. It is preferred to useorganocobalt complexes which require longer wavelengths of visible light(600-800 nm), more preferably red light (600 to 750 nm). When photolysisis utilized for the release of the bioactive agent form thebioconjugate, it is particularly preferred that the non-reactive atom ofthe bioactive agent or the atom of the spacer bound to the cobalt atombe a carbon atom.

The vitamin B₁₂ cofactor occurs naturally in two forms:adenosylcob(III)alamin, (AdoCbl^(III)), also known as coenzyme B₁₂, andmethylcob(III)alamin (CH₃Cbl^(III)). The remarkably weak C—Co bond fromthe corrin ring to the 5′-deoxyadenosyl or methyl ligand imparts a mostunusual chemistry to cobalamins. The C—Co bond energy in AdoCbl^(III)and CH₃Cbl^(III) is estimated to be as low as 31 and 37 kcal/mole,respectively. This makes the C—Co bond one of the weakest covalent bondsknown and allows photocleavage of the bond by visible light. The initialproduct of the photocleavage of AdoCbl^(III) is the geminate radicalpair {CH₂-Ado: Cbl^(II)}. Brackets {: } indicate the radical pair isgeminate (born of the same parent molecule) and held in close proximityby solvent interactions. Picosecond laser flash photolysis experimentsof AdoCbl^(III) have shown this to be a reversible process with ageminate recombination rate constant of k_(rec)≈1×10⁹ s⁻¹, followingphotolysis. Recent nanosecond laser flash photolysis studies have probeda slower radical pair recombination that occurs in the solvent and islimited by diffusion.

The π-π* electronic transition of the corrin ring of cobalamin producesa long wavelength absorption maximum at 525 nm, as shown by FIG. 1.Irradiation of alkylcobalamins at this wavelength leads to cleavage ofthe C—Co bond with a photolysis quantum yield of 0.1-0.3. The in vivophotolysis of bioconjugates according to the present invention ispreferably accomplished by delivering the light with a fiber optic probebecause of the strong absorption of hemoglobin near this wavelength. Toavoid potential problems with the absorption spectrum of cobalamin whilemaintaining a photolabile C—Co bond, Co[SALEN], which is afive-coordinate analogue of coenzyme B₁₂, can be used. Alkyl-Co[SALEN]complexes have absorption maxima near 650 nm, with significantabsorption beyond 700 nm as shown by FIG. 2. This is a distinctadvantage for photoactivatable drug release, as human tissue becomesincreasingly transparent above 610 nm. Other synthetic or naturallyoccurring B₁₂ derivatives are, or may become, available that haveabsorption maxima above 600 nm. For example, a green cobalt corrinoidhaving an absorption maximum at about 624 nm is reported by Brown et al.(1996). Human tissue becomes transparent to depths of up to about 5.7 cmat wavelengths of between about 600 and 800 nm. The use of longerwavelengths enables the more selective irradiation of limited portionsof a subject's body, with consquent release in a small target region.

The bioconjugates of the present invention can undergo cleavage byphotoactivation or photolysis in the presence of a magnetic field asfollows. The use of the magnetic field further limits the release of thebioactive agent to the desired site. For example, because of thetoxicity of antineoplastic agents to healthy tissues, it is incumbentupon any effective site specific delivery system to limit damage tocells other than at the site of activity. In the photohomoylsis cleavagereaction, the electrons in the broken covalent bond start out with theirspins paired ↑⇓ (singlet state) from having participated in the covalentbond. During the early lifetime of the radical pair, the spins retaintheir original orientation R↑+⇓R′ until the electron spins randomizeover time. During the time the spins are paired, the radicals canrecombine and revert to the starting material. If either of these pairedradical spins should intersystem cross (ISC) to the triplet spin state(R↑+⇓R′) they can no longer recombine until their spins are once againpaired. That situation is preferred to release the bioactive agent fromthe bioconjugate. However, in healthy tissue, it is desired to prevent,or at least minimize, the cleavage of the conjugate. It that event, itis desirable to alter the rate of ISC by introducing an externalmagnetic field that increases the gap between the triplet state energylevels, thus encouraging the recombination of the original bioconjugatein the healthy tissues.

The recombination rate can be increased by application of a magneticfield in the range of 100-3000 gauss to the healthy tissues leading to anet decrease in the photochemical quantum yield and a decrease in drugrelease into healthy tissues by a factor of at least 2. Application ofabout 300 to 1000 gauss is considered to be optimal. See, Grissom(1995).

The bioconjugates of the present invention can undergo cleavage byultrasound or sonolysis as follows. Although any non-reactive atom canbe bound to the cobalt atom in the bioconjugate and cleaved bysonolysis, it is preferred that the atom be a carbon atom. The vitaminB₁₂ cofactor occurs naturally in two forms: adenosylcob(III)alamin,(AdoCbl^(III)), also known as coenzyme B₁₂ and methylcob(III)alamin,(CH₃Cbl^(III)). The remarkably weak C—Co bond from the corrin ring tothe 5′-deoxyadenosyl or methyl ligand imparts a most unusual chemistryto cobalamins. The C—Co bond energy in AdoCbl^(III) and CH₃Cbl^(III) isestimated to be as low as 31 and 37 kcal/mole, respectively. This makesthe C—Co bond one of the weakest covalent bonds known and allowssonolysis of the bond by the application of ultrasound in the range ofabout 20 kHz-500 MHz, preferably 20 kHz-100 MHz, more preferably 20 kHzto 10 MHz.

Sonolysis of aqueous solutions produces a high concentration of hydroxylradicals and hydrogen atoms according to the equation:

H—OH→H.+.OH

These reactive oxidizing and reducing species are responsible forinitiating most reactions in aqueous solvents. Ultrasound irradiationand sonochemistry are often not described as high-energy processes, butduring sonolysis, the development, growth and implosion of bubbles in aliquid create extreme reaction environments on a microscopic scale. Thecollapse of cavitation bubbles produces pressures >500 atm. andtemperatures >5000° C. The radicals formed survive the collapse of thebubbles. The formation of hydroxyl radicals in vivo has been the focusof several investigations because of the potentially deleterious effectsof oxidizing free radicals in human tissue. However, such radicals donot present an unacceptable health risk, as clinical experience hasdemonstrated that diagnostic ultrasound is a benign procedure. Theability to form these radicals by sonolysis in vivo provides a mechanismfor the triggered release of active neoplastic and other agents fromconjugation with B₁₂, Co[SALEN] or other suitable cobalamin or cobalaminlike substrates.

In the sonolysis of drug-B₁₂ conjugates (utilized here only as anexample and not intended to limit the invention), the C—Co bond iscleaved in aqueous solution to produce the drug and a cob(II)alamin(Cbl^(II)) under anaerobic conditions or the drug and aquocob(III)alamin(H₂O-Cbl^(III)) under aerobic conditions. The cleavage is not a directbreaking of the C—Co bond. Rather, under anaerobic conditions thepredominant pathway for C—Co bond cleavage by sonolysis is throughreduction of drug-Cbl^(III) to the labile drug-Cbl^(II) by H., followedby dissociation to the closed-shell drug and Cbl^(II). The reaction ofHO. with drug-Cbl^(II) leads to H₂O—Cbl^(III), as well as degradation ofthe corrin ring. Under aerobic conditions, the pathway for the C—Co bondcleavage by sonolysis is through reduction of the drug-Cbl^(III) toproduce drug and Cbl^(II), but O₂ oxidizes Cbl^(II) to H₂O—Cbl^(III). Ineither event, sonolysis from the focused application of ultrasound,results in C—Co bond cleavage and the irreversible release of the drugfrom the cobalamin. Therefore, sonolysis-triggered release can occurunder aerobic and hypoxic conditions alike.

The present invention is useful in the treatment of (including, but notlimited to) cancer, hepatitis, psoriasis and other localized diseases,as well as for gene therapy and peptide therapy. The bioconjugatesaccording to the present invention can be administered by any route,including intravenously, parenterally, orally, intramuscularly,intrathecally or as an aerosol. The mode of delivery will largely dependon the biological effect desired to be achieved. A skilled artisan willreadily know the best route of administration for a particular treatmentin accordance with the present invention. The appropriate dosages willdepend on the route of administration and the treatment indicated, andcan be readily determined by a skilled artisan, such as by extrapolationfrom current treatment protocols. If the organocobalt complex of thebioconjugate is cobalamin or derivative or analogue, it is preferred toadminister orally a bolus of vitamin B₁₂ prior to administration of thebioconjugate to reduce or eliminate potential hepatotoxicity which mightotherwise result from the administration of the bioconjugate. The oraldose of B₁₂ will saturate the enterohepatic shuttle system and loadhepatocytes with cobalamin. It is preferred that 0.1 mg to 100 mg, morepreferably 1.0 mg to 10 mg, of vitamin B₁₂ be administered prior to theadministration of the bioconjugate containing cobalamin. In addition,vitamin B₁₂ can be administered, preferably intravenously, following theselective cleavage of bioconjugate to wash out all bioconjugate whichhas not been cleaved, and thus further reduce potential systemiceffects. It is preferred that 0.1 mg to 100 mg, more preferably 10 mg to100 mg, of vitamin B₁₂ in saline be administered intravenously over 4-5hrs. Finally, prior to the administration of a cobalamin-basedbioconjugate, nitrous oxide can be administered to the subject in orderto deplete body stores of cobalamin in its various forms, such asmethylcobalamin. Administration of nitrous oxide has the effect ofcreating a greater body deficit of cobalamin before administration ofthe cobalamin-based bioconjugate.

Pharmaceutical compositions containing a compound of the presentinvention as the active ingredient can be prepared according toconventional pharmaceutical compounding techniques. See, for example,Remington's Pharmaceutical Sciences, 17th Ed. (1985, Mack PublishingCo., Easton, Pa.). Typically, an antagonistic amount of activeingredient will be admixed with a pharmaceutically acceptable carrier.The carrier may take a wide variety of forms depending on the form ofpreparation desired for administration, e.g., intravenous, oral,parenteral, intrathecal, transdermal, or by aerosol.

For oral administration, the compounds can be formulated into solid orliquid preparations such as capsules, pills, tablets, lozenges, melts,powders, suspensions or emulsions. In preparing the compositions in oraldosage form, any of the usual pharmaceutical media may be employed, suchas, for example, water, glycols, oils, alcohols, flavoring agents,preservatives, coloring agents, suspending agents, and the like in thecase of oral liquid preparations (such as, for example, suspensions,elixirs and solutions); or carriers such as starches, sugars, diluents,granulating agents, lubricants, binders, disintegrating agents and thelike in the case of oral solid preparations (such as. for example,powders, capsules and tablets). Because of their ease in administration,tablets and capsules represent the most advantageous oral dosage unitform, in which case solid pharmaceutical carriers are obviouslyemployed. If desired, tablets may be sugar-coated or enteric-coated bystandard techniques. The active agent must be stable to passage throughthe gastrointestinal tract. If necessary, suitable agents for stablepassage can be used, and may include phospholipids or lecithinderivatives described in the literature, as well as liposomes,microparticles (including microspheres and macrospheres).

For parenteral administration, the compound may dissolved in apharmaceutical carrier and administered as either a solution or asuspension. Illustrative of suitable carriers are water, saline,dextrose solutions, fructose solutions, ethanol, or oils of animal,vegetative or synthetic origin. The carrier may also contain otheringredients, for example, preservatives, suspending agents, solubilizingagents, buffers and the like. When the compounds are being administeredintracerebroventricularly or intrathecally, they may also be dissolvedin cerebrospinal fluid.

In the treatment of cancer (sarcomas, carcinomas or leukemias), adistinction can be made between the types of systemic adjuvantchemotherapies that are typically used in concert with the more extrememethods of surgical excision and radiation therapy. There are two broadclasses of chemotherapeutic adjuvants: (1) endocrine and antivasogenictherapeutic agents which are aimed at altering the body's physiology;and (2) cytotoxic chemotherapeutic agents which are typicallyadministered systemically to kill or inhibit the growth of transformedcells.

Cytotoxic or antineoplastic agents are represented by a number of drugclasses. Alkylating agents undergo chemical reactions that generatehighly reactive electrophilic carborationsnium ions that readily formcovalent linkages (alkylate) with various nucleophilic biologicallyimportant moieties such as nucleic acid bases and phosphate, amine,sulfhydryl, hydroxyl, carboxyl and imidazole groups. These agents, amongother functions, have cytotoxic actions that disturb the fundamentalmechanisms concerned with cell growth, mitotic activity, differentiationand function. Chlorambucil, modified busulfan, cyclophosphamide,ifosfamide and cisplatin and its structural analogs are representativealkylating agents.

Antimetabolites such as folic acid analogs (e.g. methotrexate) andpyrimidine analogs (e.g. fluorouracil and fluorodeoxyuridine) exertcytotoxic activity by blocking or preventing metabolic pathways leadingto neoplastic cell destruction. Methotrexate is also known to be usefulin the treatment of psoriasis by inhibiting the proliferation ofepidermal cells.

Another potent cytotoxic class is mitotic inhibitors such as thepaclidaxel or the alkaloids camptothecin, vincristine and vinblastine.

Also, certain antibiotics, such as doxorubicin and daunorubicin,(tetracyclic aglycone glycosides), intercalcate with DNA and inhibitnucleic acid synthesis.

In accordance with the present invention, bioconjugates for thetreatment of cancer are formed preferably using chemotherapeuticsselected from the group consisting of alkylating agents, antimetabolitesand mitotic inhibitors. For example, methotrexate is an antimetabolite;chlorambucil, cisplatin and modified busulfan are alkylating agents, andcamptothecin and its derivatives are alkaloids. The bioconjugates formedfrom these cytotoxic agents can be administered intravenously for thetreatment of the specific classes of cancer for which they are known tobe effective, e.g. cancer of the colon, lung, kidney, breast, prostate,melanoma, nasopharyngeal, T-cell leukemia, myelogenous leukemia,lymphocytic leukemia and the like. When delivered intravenously to theblood stream and the bioconjugates contain cobalamin, the naturalaffinity of cancer cells for B₁₂ will target the bioconjugates to thesetissues or cell sites. Alternatively, the bioconjugates can beengineered to be selective for the delivery of the chemotherapeuticagent to the desired cancer cell by the incorporation of a suitabletargeting molecule (such as those set forth above) on the organocobaltcomplex.

In accordance with the present invention, solid tumors are treated asfollows, with use of a drug-B₁₂ bioconjugate as an example. This exampleis not intended to limit the present invention in any manner, and askilled artisan could readily determine other bioconjugates of thepresent invention which could be utilized for the treatment of solidtumors. The drug-B₁₂ bioconjugate is administered, preferablyintravenously, to a cancer patient to target metastatic cancer when thecancer cell has a significant requirement for cobalamin. This propensityof cobalamins to migrate to the cancer cells significantly reducescardiotoxicity, myelotoxicity, hepatotoxicity and similar side effectsthat limit the size and frequency of effective dosing of antineoplasticagents. Furthermore, problems associated with toxicity to non-targetedcells is minimized. Delivery is further enhanced by the triggering ofthe release of the antineoplastic agent from the bioconjugate by themechanism of photolysis or sonolysis which provides for a high degree ofspatial and temporal control of the drug release at a localized areaover a short time. The application of a magnetic field with photolysisfurther serves to protect health cells by recombination of thebioconjugate and limit the release of active agent to the specificcancer cell-containing site(s).

Although chemotherapy is generally reserved for targeting metastasizedcells after the surgical excision of the primary tumor mass, thetriggered release of a bioactive agent drawn to the tumor site allowsfor treatment of the primary tumor, as well as metastatic neoplasms thathave spread to a limited and known area. The bioconjugate dosage, lengthof treatment, degree of photoactivation, and other treatment parameterscan be determined by one skilled in the art based on the type of cancer,antineoplastic agent administered, specific cobalamin used, condition ofthe patient and other factors which are variable and best determined ona case-by-case basis.

In accordance with the present invention, leukemia is treated asfollows, with use of a drug-B₁₂ bioconjugate as an example. This exampleis not intended to limit the present invention in any manner, and askilled artisan could readily determine other bioconjugates of thepresent invention which could be utilized for the treatment of leukemia.At least two forms of leukemia, chronic myeloid leukemia (CML) and acutepromyeloctyic leukemia (APL), produce high levels of B₁₂ bindingproteins that result in a 3- to 36-fold increase in the unsaturated B₁₂binding capacity in the blood. The increased concentration of B₁₂binding proteins is consistent with the rapid turnover of immature bloodcells and provides an opportunity to target the delivery of antileukemicdrugs, such as bis-alkylating agents derived from busulfan, to thetransformed hematopoietic cells responsible for the leukemic condition.The bioconjugates of this invention provide a means for the effectiveadministration of such alkylating agents to cell sites from which theactive agent can be released from the conjugate. This targeted deliveryand release provides a significant advance in the treatment of CML andAPL, for which current chemotherapy methods sometimes provide incompleteremission.

The present invention is also useful for the treatment of psoriasis.Psoriasis is a prime target for the transdermally or orally controlleddelivery of antimetabolites activated by photolytically inducedcleavage. Although not life-threatening, psoriasis can significantlydiminish the quality of life of patients who experience severeexfoliation associated with psoriatic and rheumatoid arthritis.Antimetabolites, such as methotrexate and 5-fluorouracil, are effectivein controlling severe cases of skin proliferation. Effective oralTherapy is limited by hepatotoxicity in spite of low dosing, and therisk of cumulative liver damage requires such therapy to be reserved foronly the most severe episodes during a patient's life. The delivery ofsuch agents to the skin improves the appearance and psychologicalquality of life of patients whose lives have been dominated by severepsoriasis.

The present invention is further useful for peptide therapy. One exampleof peptide therapy is as a cytotoxic agent, for example as anantineoplastic agent. In this example, a bioconjugate containing theenzymatic domain of diphtheria toxin (DT) is administered to a subjectsuch as described above for solid tumors or leukemia. The targetedrelease of the DT peptide results in the inhibition of protein synthesisand eventual cell death.

The present invention is also useful for gene therapy. One example ofgene therapy is the delivery of an antisense oligonucleotide to inhibitviral gene expression and viral replication. In this example, abioconjugate containing an antisense oligonucleotide against hepatitis Bvirus is administered to a patient having a hepatitis B infection. Theaccumulation of the bioconjugate and release of the antisenseoligonucleotide in the liver inhibits hepatitis B virus gene expressionand replication.

The present invention is further described by reference to the followingExamples, which are offered by way of illustration and are not intendedto limit the invention in any manner. Standard techniques well known inthe art or the techniques specifically described below were utilized.

EXAMPLE 1 Photolysis of B₁₂ and Co[SALEN] Bioconjugates

A protocol for a typical anaerobic continuous-wave photolysis ofmethylcob(III)alamin or CH₃Cbl^(III) is as follows. An aqueous solutionof 200 μM CH₃Cbl^(III) and 50 mM K⁺ Hepes pH 7.3 is placed in a 1 cmquartz cuvette. Samples that are to be photolyzed under anaerobicconditions are either subjected to repeated freeze-pump-thaw cycles, orpurged with Ar for 40 minutes immediately prior to photolysis andsealed. Continuous-wave visible light irradiation is accomplished at thedesired wavelength with an Ar⁺ pumped dye laser. The incident light onthe face of the cuvette is reduced to 12 mW cm⁻² with neutral densityfilters. The light flux is determined by potassium ferrioxalateactinomety and by a Scientech surface-reading thermopile. The cuvette isplaced in a thermostated cell holder at 25-37° C. For quantum yieldmeasurements as a function of magnetic field, the cuvette is placed inthe gap of a GMW Associates electromagnet with 7.5 cm diametercylindrical poles. The magnetic field within the area of the cuvette ishomogeneous to within 2% and the long term stability is better than 0.5%as monitored by a transverse Hall probe and digital teslameter.

Absorption spectra from 300-600 nm are recorded in one second with adiode array spectrophotometer at variable time intervals from 10 secondsto 2 minutes (depending upon the fluence of the photolyzing lightsource) for a total of 3τ_(½). Exposure to light during analysis is keptto a minimum. The concentration of CH₃Cbl^(III) is determined using themeasured absorbance at 350 or 520 nm by the method of Chen and Chance(1993). The plot of [CH₃Cbl^(III)] vs. time (t) appears zero-order inall cases.

Selection of Photolysis Wavelength:

The absorption spectra for CH₃Cbl^(III) and ethyl-Co[SALEN] are shown inFIGS. 1 and 2. For CH₃Cbl^(III) the π-π* electronic transitions thatlead to cleavage of the C—Co bond are maximal at 377 and 528 nm. Muchpreliminary work with B₁₂ photolysis has been carried out with 514 nrilight from an Argon-ion (Ar⁺) laser. This is close to thelong-wavelength maximum absorbance and gives a quantum yield of about0.3 for CH₃ Cbl^(III).

The absorbance of blood and tissue is significant at this optimalwavelength for cob(III)alamin excitation. Blood has a low (partial)transmittance window near 514 nm. This absorbance is sufficient toquickly pyrolize whole bovine blood placed in the light path of a 20W/cm² beam of 514 rim light.

It would therefore be beneficial to provide a cobalamin for conjugationwherein the π-π* electronic transitions that lead to cleavage of theC—Co bond are maximal at a wavelength where there is minimal or nointerference. Above about 610 nm blood becomes partially transparent andlosses beyond 50% transmittance are largely due to light scattering fromthe erythrocytes. Heparinized bovine blood placed in the light path of a20 W/cm 2 beam of 630 nm light shows only minor heating over longexposure times. There is also demonstrated a high transmittance oftissue at 610-800 nm.

This suggests the use of an organocobalt complex for conjugation havingan absorption wavelength where tissue and blood are relativelytransparent. FIG. 2 shows that ethyl-Co[SALEN] complexes have absorptionmaximums near 650 nm, with significant absorption beyond 700 nm. AnAr⁺-pumped dye laser or a Krypton-ion (Kr⁺) laser can be a suitablehigh-intensity source of photons in the region of 610 nm. Ar⁺-pumped dyelasers are often used for photodynamic therapy with hematoporphyrins.Also, an inexpensive He—Ne laser, having a principal line at 633 nmmight be used. However, such lasers are typically limited to 50 mWmaximum output.

There are laser dyes in the 600-700 nm region that can achieve energyconversion efficiencies of up to 45%. This means that a 6 W Ar⁺ pumplaser can yield nearly 3 W of spatially-coherent monochromatic light inthe region of 610-750 nm. The exact wavelength can be chosen to optimizethe continuous-wave quantum yield and still maintain a reasonable degreeof tissue penetration. In tests with alkyl-Co[SALEN] complexes it hasbeen found that 690 nm light from an Ar⁺-pumped dye laser operating withrhodamine 6-G dye is satisfactory. Optimization can be determineddepending on the specific cobalamin chosen for animal and/or clinicaltrials of the bioconjugates. In addition, high-power diode lasers thatemit red light of the desirable wavelength are commercially available.These diode lasers have the advantage of providing up to 100 watts ofoptical power in a narrow region of the optical spectrum that is usefulfor triggering cleavage of the bioconjugates.

EXAMPLE 2 Sonolysis of B₁₂ and Co[SALEN] Bioconjugates

Sonolysis was carried out with a Branson ultrasonic bath (model 3200)operating at 47 kHz. The correct placement of the reaction vessel at afocal point of high-intensity ultrasound was determined by the oxidationof iodide to iodine in the presence of starch (Mason, 1991) and thetemperature of the bath was maintained at 21° C. by a constanttemperature circulator. Aerobic sonolysis was typically carried out in atest tube or Erlenmeyer flask, whereas anaerobic sonolysis was carriedout in a closed reaction vessel fitted with a sidearm and quartzcuvette. Anaerobic conditions were produced by sparging with Ar for 30min prior to sonolysis. In some experiments, the pH was buffered by theuse of 100 mM phosphate (aerobic experiments) or 100 mMN-(2-hydroxyethyl)piperazine-N-2-ethanesulfonate (Hepes) (anaerobicexperiments), as specified. All procedures were carried out in theabsence of light to prevent photolytic cleavage of the Co—C bond.Absorption spectra were recorded on a diode array spectrophotometer (HP8452A). The solutions were transferred to a quartz cuvette with a 1 cmlight path for all optical measurements and care was exercised to ensurethat insignificant photolysis occured during the 1 s measurement time.

Sonolysis of Methylcob(III)alamin:

Sequential absorption spectra of aqueous CH₃—Cbl^(III) as a function ofanaerobic sonolysis (pH 7.38, 100 mM Hepes, saturating Ar) are shown inFIG. 3A. The absorbance at 340, 374, and 520 nm decreases linearly as afunction of sonolysis time and the absorbance at 316 and 420 nmincreases linearly, thereby indicating the reaction is zero order insubstrate concentration. The isosbestic points at 336, 390, and 585 nmare in agreement with those obtained through anaerobic photolysis ofCH₃—Cbl^(III). Under the conditions of sonolysis, an additionalisosbestic point occurs at 476 nm, rather than at 486 nm, as typicallyobserved in the course of photolysis. This slight shift in theisosbestic point is caused by a minor product that has an absorbancemaximum near 490 nm. This may be cob(I)alamin that has a sufficientlifetime (t_(½)=22 min at pH 6) to be observed spectrophotometrically.The absorption band at 374 nm is characteristic of a C—Co bond, and itsdisappearance unambiguously indicates displacement of the axial carbonligand.

Under aerobic conditions, molecular oxygen scavenges H. and prevents thereduction of CH₃—Cbl^(III) via the equation

O₂+H.→.OOH

In the absence of an organic buffer with abstractable hydrogen atoms,reaction via HO. remains to be a viable process.

FIG. 3B shows the change in absorbance spectra following aerobicsonolysis in the absence of organic buffer. The decrease in absorbanceat 340 and 374 nm is linear with increasing sonolysis time indicatingthe reaction is zero order in substrate concentration, but theunexpected increase in absorbance at 520 nm indicates the stable productof cobalamin sonolysis is not hydroxocob(III)alamin, as would beexpected if molecular oxygen were to reoxidize cob(II)alamin tocob(III)alamin. Aerobic photolysis under the same conditions shows theexpected decrease in absorbance at 374 nm but no change at 520 nm. Thisdifference suggests that HO. is able to displace the alkyl ligand fromCol^(III), but other HO. reactions also occur (perhaps through thesecondary products HOO. and.O₂ ⁻) to oxidize the corrin ring. Similarabsorbance spectra are obtained from sonolysis of an aerated aqueoussolution containing 100 mM phosphate buffer.

A similar result is seen in the reaction of CH₃—Cbl^(III) with H. andHO. when these radical species are generated by pulse radiolysis(Blackburn et al., 1972). Reducing species H. reacts to produce the samespectral changes as shown in FIG. 3A. Multiple oxidizing species (HOO.and O₂ ⁻) can react with CH₃—Cbl^(III) to cleave the Co—C bond, butthese species also lead to the irreversible degradation of the corrinring, as evidenced by the spectral changes similar to those seen in FIG.3B. A precedent for irreversible oxidation of the corrin ring exists inthe photooxygenolysis of alkylcobalamins by singlet oxygen (Krautler andStepanek, 1985).

Aerobic sonolysis of solutions containing 100 mM Hepes or 100 mM t-butylalcohol produces no change in the absorption spectra over comparabletime. This is because molecular oxygen quenches the H. reaction pathway,and t-butyl alcohol quenches the HO. reaction pathway. Although Hepeshas not previously been reported to be a scavenger of HO., many reportsindicate that organic solute molecules such as formate, can inhibit thereaction of HO. (Weissler, 1962). The absence of any spectral changesunder these conditions suggests that direct sonolysis of the Co—C bondis not an important reaction pathway.

EXAMPLE 3 Biological Testing Against NCI Human Tumor Cell Lines

The efficacy of the bioconjugates is tested against tumor cell linesusing existing protocols for assessing the effectiveness of targetedcoenzyme B₁₂ antineoplastic agent-containing bioconjugates.Representative cell lines tested include HCT 116 (human colon tumor),A549 (human lung), ACHN (human kidney), MCF7 (human breast), humanprostate, SK5-mel (human melanoma), KB (human nasopharyngeal), CCRF-CEM(human T-cell leukemia), HL-60 (human promyelocytic leukemia), RD-995(mouse fibrosarcoma), B-16 (mouse melanoma) and Meth-A (mousecarcinoma). Drug screening is carried out with a calorimetric cellviability assay in a 96-well plate.

Additionally, selected bioconjugates are radiolabeled with ¹⁴C or ³H toassess the level of uptake by human tumor cells. As noted above, theprior art reports that some tumor and leukemia cells produce high levelsof B₁₂ binding proteins in the serum and sequester B₁₂ in highconcentrations of up to 50 fold.

Tumor Cell Line Testing Protocol:

The drug bioconjugates, synthesized under procedures described herein oradaptions thereof, are diluted over 5 orders of magnitude (approximately0.005 to 50 μg/mL). Four hours after seeding of the cell in the plate,the cells are treated with the appropriate drug dissolved in isotonicbuffered solution. In control experiments, without photolysis triggereddrug release, the drug is left on the cells for three days, as in thenormal basic cancer screen. In the wells for triggered drug release, thelaser output is focused on selected wells for varying times and withvarying intensity. Alternatively, a matrix of light-emitting diodes(LEDs) is used. The cells are incubated under standard mammalian tissueculture conditions under the proper CO₂ balanced atmosphere. After threedays, the cells are re-fed and the calorimetric dye3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetra-zolium bromide (MTT) isadded. The reduction of the MTT to purple formazan product is quantifiedin a 96 well plate reader. The concentration of the purple formazan dyeis correlated with the number of viable cells. The reduction in cellsurvival at a given dose rate and photolysis exposure give aquantitative estimation of cell death and drug delivery effectiveness.Care is taken not to expose portions of the plate to photolysisconditions through adventitious spillover of radiation. This isaccomplished by using 96 well plates with an opaque mask (Fisher cat#07-200-565) for photolysis.

Uptake of Drug-B₁₂ and Drug-Co[SALEN] Bioconjugates:

The uptake of drug-B₁₂ and drug-Co[SALEN] bioconjugates by culturedtumor cells is monitored by radiolabeling the drug or cobalamin duringsynthesis. ³H-Labeled 5-fluorouracil, methotrexate and chlorambucil arepurchased from DuPont/NEN (New England Nuclear). These drugbioconjugates, as well as ¹⁴C-labeled methylcob(III)alamin (synthesizedfrom Cob(I)alamin and ¹⁴CH₃I) provide an indication of receptor-mediateduptake by the various tumor cell lines. In this study, the cells areexposed to the radiolabeled drug as described in the preceding section,except no MTT is added at the end of the three-day incubation period.Since all of the cell lines except the leukemia cells grow whileattached to the bottom of the microtiter plate well, the growth mediumis aspirated to remove the unincorporated radiolabeled drug, followed byseveral washes with fresh medium. The labeled cells are detached fromthe bottom of the wells and the radioactivity quantified byscintillation counting. Growth of non-attached leukemia cell takes placein round-bottom microtiter plates such that centrifugation sediments thecells and allows washing with fresh growth medium before solubilizingthe cells and quantifying the incorporated radiolabeled drug byscintillation counting.

EXAMPLE 4 Synthesis of Co[SALEN]

Synthesis of N,N′-bis(salicylidene)ethylenediamine.

To a stirred solution of salicylaldehyde (12.21 g/10.62 mL) in 70° C.ethanol (100 mL) was added ethylenediamine (3.01 g/3.33 mL). A yellowcrystalline material immediately formed, and the reaction mixture wasallowed to cool to room temperature with stirring. The solution wasfiltered, and the crystals were washed with cold ethanol. The ethanollayers were ombined and reduced to approximately 20 mL and allowed tostand at 0° C. overnight. The resulting crystals were collected byvacuum filtration and washed with water. The collected solids were driedin vacuo to obtain 13.15 g (98%) N,N′-bis(salicylidene)ethylenediamineas yellow platelets with a melting point of 126° C. (literature value(24) 127-128° c.). ¹H NMR (DMSO-d₆) δ 8.57 (s 2H, HC=N), 7.42 (d, 2Haromatic, J=7.3 Hz), 7.31 (t, 2H, aromatic, J=9.0 Hz), 6.88 (t, 4H,aromatic, J=8.3, 16.1 Hz), 3.89 (s, 4H, CH₂). ¹³C NMR (DMSO-d₆) δ 166.94(2C, CO⁻), 160.57 (2C, HC+N), 132.38 (2C, aromatic), 131.69 (4C,aromatic), 118.59 (2C, aromatic), 116.51 (2C, aromatic), 58.88 (2C,CH₂).

Synthesis of N,N′-bis(salicylidene)ethylenediaminecobalt(II)(Co[SALEN]).

To a hot (100° C.) deoxygenated solution of the above product (2.68 g)in dimethylformamide (25 mL) was added via cannula needle an aqueoussolution (10 mL) of cobalt (II) acetate tetrahydrate (2.49 g). The redprecipitate which formed was collected by vacuum filtration, washed withcold dimethylformamide, and dried in vacuo to obtain 2.6 g (80%) ofN,N′-bis-(salicylidene)ethylenediaminocobalt (II) as red crystals.

EXAMPLE 5 Synthesis of Modified Co[SALEN]

The diglycolate ether of Co[SALEN] is prepared as described in Example4, using the glycolate ether of 2,5-dihydroxybenzaldehyde in place ofsalicylaldehyde. An unsymmetrically substituted (glycolate ether/amide)complex is prepared as described in Example 4 by usi g a mixture of theglycolate ether of 2,5-dihydroxybenzaldehyde and 5-aminosalicylaldehydein place of salicylaldehyde.

EXAMPLE 6 Synthesis of Chlorambucil-Cobalamin Bioconjugates

Synthesis of1-bromo-2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethane.

Twenty-five mL of freshly distilled CH₂Cl₂, 0.343 gdicyclohexylcarbodiimide (1.66 mmol), 0.305 g 4-dimethylaminopyridine(2.5 mmol), and 0.263 g 4-dimethylamino-pyridinium chloride (1.66 mmol)were added to a flame-dried 50 mL round bottom flask equipped with astir bar, reflux condenser, and Ar inlet (Boden and Keck, 1985). Thesolution was purged with argon and brought to reflux. While refluxing, asolution of 0.304 g chlorambucil (1.0 mmol) and 0.125 g 2-bromoethanol(1.0 mmol) in 5 mL CH₂Cl₂ (purged under argon for 30 min.) wastransferred via cannula to the refluxing solution over a period of 30min. After addition was complete, the reaction mixture was stirred for 2h at room temperature. Precipitated dicyclohexylurea was removed byfiltration and the solution was concentrated by rotary evaporation. Theresulting residue was taken up in CH₂Cl₂, filtered, and purified byflash silica column chromatography. The desired product was eluted using1:9 ethyl acetate:hexanes (v/v) to give 0.374 g of a yellow oil in 91%yield (ester 2). ¹H NMR (CDCl₃, 300 MHz) d 7.06 (d, 2H, J=8.4 Hz), 6.60(d, 2H, J=8.7 Hz), 4.35 (t, 2H, J=6.15 Hz), 3.56-3.72 (m, 8H), 3.48 (t,2H, J=6.15 Hz), 2.56 (t, 2H, J=7.65 Hz), 2.35 (t, 2H, J=7.35 Hz), 1.91(quintet, 2H, J=7.58 Hz). ¹³C NMR (CDCl₃, 75 MHz ¹H decoupled) d 173.05,144.35, 130.37, 129.75 (2), 112.12 (2), 63.68, 53.55 (2), 40.60 (2),33.94, 33.41, 29.05, 26.72.

Synthesis of2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethylcob(III)alamin(3).

Two hundred mg of hydroxocob(III)alamin (0.15 mmol) was dissolved in 10mL water and purged with Ar while stirring (Brown and Peck, 1988). Theexiting gas was conducted in sequence through: (1) a flask containing0.025 g NaBH₄ (0.66 mmol); (2) a flask containing 5 mL H₂O; and (3) aflask containing 0.226 g ester 2 (0.55 mmol) in 5 mL CH₃OH. Afterdeaerating for 1 h, the water from flask (2) was transferred to flask(1) containing NaBH₄ via canrula and swirled to promote dissolution.This solution was transferred via cannula to the aqueous cobalaminsolution. Reduction was allowed to proceed for 20 min, after which thechlorambucil bromoethylester was added to the solution. The reactionmixture was allowed to stir for an additional 5 min. and then 0.2 mLacetone was added to destroy the excess borohydride. The solution wasconcentrated to approximately 2 mL by rotary evaporation and theresulting solution was applied to a 2.5×30 cm column of Amberlite XAD-2resin. The column was washed with 1 L H₂O to desalt and the coba aminwas eluted with 50% aqueous acetonitrile (v/v). The eluent was reducedto approximately 2 mL by rotary evaporation and the solution was appliedto a 1×40 cm column of SP-Sephadex C25 (Na⁺ form). Elution with waterremoved the major red band which was reduced to a minimal volume.Acetone was added until faint turbidity persisted after swirling. Thesolution was allowed to stand for 1 h at 0° C. and excess acetone wasadded to promote further crystallization. The crystals were collected byvacuum filtration and dried in vacuo. 3 was obtained as red crystals(122.5 mg) with a yield of 53%. MS (FAB+) calcd for C₆₈H₁₁₂N₁₄O₁₆CoPCl₂, 1541; found 1541.

4-[4′-(bis-[2-chloroethyl]amino)phenyl]butyroylcob(III)alamin (4) wassynthesized in a similar manner starting with the acid chloride ofchlorambucil and reating it with hydroxocob(III)alamin as above.

Synthesis of2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethyl-Co[SALEN] and4-[4′-(bis-[2-chloroethyl]amino)phenyl]butyroyl-Co[SALEN] are synthsizedin a similar manner using Co[SALEN] in place of hydroxocob(III)alamin.

Synthesis of2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethyl-(Co[SALEN]-folate)and 4-[4′-(bis-[2-chloroethyl]amino)phenyl]butyroyl-(Co[SALEN]-folate)are synthsized in a similar manner using Co[SALEN]-folate in place ofhydroxocob(III)alamin.

Synthesis of2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethyl-(greencorrinoid) and 4-[4′-(bis-[2-chloroethyl]amino)phenyl]butyroyl-(greencorrinoid) are synthsized in a similar manner using CH₃-Co(III)corrinoid (prepared by reacting methyliodide with the green corrinoid ofBrown et al. (1996) after it had been reduced with NaBH₄) in place ofhydroxocob(III)alamin.

EXAMPLE 7 Sonolysis of2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butoxy]ethylcob(III)alamin (3)

The products released by exhaustive sonolysis, as described in Example2, of compound 3 (prepared in Example 6) were isolated by reverse-phaseHPLC (Rainin Microsorb C-18). Elution and separation of the sonolysisproducts were carried out with an increasing gradient of acetonitrile(A) and 0.05 M phosphoric acid (B): initial condition 5% A: 95% B,increased linearly for 10 min to 30% A and 70% B, maintained for 2 min;followed by a linear increase to 70% A and 30% B over 10 min (Rinchik etal., 1993). The solvent was evaporated from each fraction and theproducts were extracted with CH₂Cl₂ and characterized by ¹H and ¹³C NMR.

Sequential absorption spectra of aqueous 3 as a function of anaerobicsonolysis at pH 7.4, 100 mM Hepes, saturating Ar, are shown in FIG. 4A.The absorbance at 374 and 520 nm decreases linearly as a function ofsonolysis time, and the absorbance at 316 and 420 nm increases linearly,thereby indicating the reaction is zero order in substrateconcentration. The isosbestic points at 336, 390, 486 and 585 nm are inagreement with those obtained through anaerobic photolysis ofCH₃-Cbl^(III). The absorption band at 374 nm is characteristic of a Co—Cbond, and its disappearance unambiguously indicates displacement of theaxial carbon ligand.

FIG. 4B shows the change in absorbance spectra following aerobicsonolysis of a 3 solution containing phosphate buffer. Different stableproducts are obtained under aerobic conditions. Because of the presenceof molecular oxygen, the released product was shown by NMR to be2-[4-(4′-[bis-(2-chloroethyl)amino]phenyl)butyroxy]ethan-1-al. ¹H NMR(CDCl₃, 300 MHz) 9.59 (s, 1H), 7.08 (d, 2H, J=2.9 Hz), 6.62 (d, 2H,J=2.9 Hz), 4.67 (s, 2H), 3.73-3.59 (m, 8H), 2.60 (t, 2H, J=7.5 Hz), 2.45(t, 2H, J=7.4 Hz), 1.95 (quintet, 2H, J=7.4); ¹³C NMR (CDCl₃, 75 MHz ¹Hdecoupled) 195.85, 173.09, 144.54, 130.43, 129.92 (2), 112.29 (2),68.73, 53.74 (2), 40.69 (2), 33.99, 33.13, 26.79. The decrease inabsorbance at 374 nm is linear with increasing sonolysis time indicatingthe reaction is zero order in substrate concentration.

The Co—C bond of CH₃-Cbl^(III) can be cleaved by sonolysis in aqueoussolutions to produce the alkane and cob(II)alamin under anaerobicconditions or to produce the aldehyde and hydroxocob(III)alamin underaerobic conditions. Unlike photolysis and thermolysis that lead todirect Co—C bond cleavage, the predominant pathway for Co—C bondcleavage by sonolysis is through H. mediated reduction of CH₃—Cbl^(III)to the labile 19 e⁻ CH₃—Cbl^(II−) species followed by dissociation tothe closed-shell alkane and Cbl^(II), or through the reaction of HO.with CH₃—Cbl^(III) that leads to formation of hydroxocob(III)alamin aswell as degradation of the corrin ring.

A parallel exists between the reactions of alkylcob(III)alarnin underthe conditions of sonolysis and pulse radiolysis, (Blackburn et al.,1972) but without the need for expensive equipment. Although the violentcavitation during sonolysis has sufficient energy to break the Co—C bondto produce the {R.Cbl^(II)} radical pair by a dissociative pathwayanalogous to the photolysis of CH₃—Cbl^(III), (Endicott and Netzel,1979; Chagovetz and Grissom, 1993; Natarajan and Grissom, 1996),alkylcob(III)alamins are not sufficiently volatile to be found in theextreme environment of the collapsing bubbles. Therefore, direct Co—Cbond cleavage by sonolysis is not possible in spite of the more than 80kcal/mol difference in bond-dissociation energy between Co—C and H—OH.

Anaerobic sonolysis of the Co—C bond is irreversible because aclosed-shell alkane is formed. Under aerobic conditions, the rate of H.reaction with O₂ is on the same order of magnitude as the reaction of H.with CH₃, thereby suggesting the closed-shell alkane, CH₄, should be oneof the end products of CH₃—Cbl^(III) sonolysis (Buxton et al., 1988;Baulch et al., 1992). In contrast, Co—C bond cleavage of CH₃—Cbl^(III),via anaerobic photolysis, is reversible from the {CH₃.Cbl^(II)} radicalpair.

In summary, the ability to form cob(II)alamin and the closed-shellalkane without the use of chemical reductants and without the use ofelectrochemical, photochemical, or pulse radiolysis equipment may be auseful method to promote activation of drug-cobalamin complexes in vivo.

EXAMPLE 8 Materials and Methods for in vitro Assays of BioconjugateActivity

Media Preparation

All media were purchased from Sigma and materials used to supplement themedia were purchased from Atlanta Biologicals. The HL-60 cell culturewas grown in an α-MEM media. The media was completed prior toinoculation by the addition of reagents to bring the final mediaconcentration to 7.5% w/v sodium bicarbonate, 10% fetal calf serum, 100μg/mL, penicillin and streptomycin, and 50 units/mL mystatin. McCoy'smedia, with sodium bicarbonate buffer, was used for HCT-116 cellculture. It was completed in the same manner with 8% newborn calf serumand 2% fetal calf serum. Completed media could be stored at 4° C. forseveral weeks. The culture medium was warmed to 37° C. beforeinoculation with cells. Stock Culture Preparation and Maintenance Stockcell cultures were started from ATCC cell lines. The original ATCC cellline was aliquoted in 10% DMSO and stored in liquid nitrogen. Stockcultures of 40 mL were grown and maintained in collaen-treated, sterile75 mL culture flasks purchased from Corning. The cultures were incubatedat 37° C. in a 5% CO₂ environment to maintain a pH of 7.1. Humiditywithin the incubator was maintained to prevent hypertonicity in themedia by placing an open pan of water in the bottom of the incubator.

The concentration of cells within the stock culture was controlled andcell concentrations were estimated in several ways. The media becamemore purple and subsequently orange as a result of cell metabolism andmetabolic byproducts that accumulated in the media. The cells were alsovisually observed under microscope at 40× and 100× power. Normal HCT-116cells appeared rounded, flat, and adhered strongly to the walls of theculture flask. When the cells almost covered the bottom of the flask,the cell concentration was reduced. Normal HL-60 cells appeared round,but were well differentiated and easily suspended in the media. Changesin cell morphology were often indicative of bacterial or fungalcontamination. For the accurate determination of cell concentrations, aCoulter Cell CounterTM was employed. Stock cultures were not allowed togrow to greater than 100,000 cells/mL. Both of the cell lines wereobserved to have a doubling time of about 24 hrs.

Assay Preparation

The assays were performed in collagen-treated, sterile 96-well platesthat were purchased from Corning. HL-60 cells were grown inround-bottomed wells (Coming catalog #25850) and HCT-116 cells weregrown in flat-bottomed wells (Corning catalog #25860). Cellconcentrations were measured by a Coulter Cell Counter™. Cells werediluted in bulk and loaded onto the plates with 200 μL in each well. Theassays were performed using approximately 25,000 HL-60 cells/well and40,000 HCT-116 cells/well.

Since HL-60 cells grow in suspension, the cell concentration wasmeasured and diluted directly. HCT-116 cells, however, adhere to thewalls of the flask and must be suspended by treatment with trypsin. A0.025 mg/mL trypsin solution was thawed immediately before use. The bulkculture medium was removed by aspiration and 2 mL of the cold trypsinsolution were added to the flask. The flask was agitated periodically topromote suspension of the cells. Care was taken to limit cell exposureto the trypsin solution to less than five minutes, since prolongedexposure will damage the cell membrane. When the cells were suspended,as observed by a microscope, 8 mL of media were added to inactivate thetrypsin. The cell concentration in the resulting suspension wasmeasured, the suspension diluted appropriately, and loaded onto the96-well plates. Once on the plates, the cells where allowed to adherefor 3 hrs before treatment with SFU or one of the derivatives.

Cell Growth and MTT Determination of Cell Viability

HL-60 cells were treated and placed in the incubator for 24 hrs. Theplates were centrifuged and the supernatant was carefully aspiratedwithout disturbing the cell pellet. A 200 μL aliquot of media was addedimmediately. The cells were allowed to grow for 48 hrs. HCT-116 cellswere treated and allowed to grow undisturbed for 72 hrs. The culturemedium was removed by aspiration (after centrifugation in the case ofHL-60 cells). 100 mL of McCoy's media and 11 μL MTT dye were added. Thecells were incubated at 37° C. for 3 hrs. During this time, viable cellsreduce the MTT dye to purple formazan by the action of alcoholdehydrogenase. The cells were lysed by the addition of 100 μL of asolution 1.2M Hcl in 60% ethanol, thereby releasing the reduced dye intosolution. The absorbance at 405 nm was measured for each well using aBIO-RAD Microplate Reader (Model 450™/

EXAMPLE 9 In Vitro Activity of Chlorambucil-Cobalamin Bioconjugates

Thermal Stability of Bioconjugates in Media

It was noted that the chlorambucil bioconjugates 3 and 4 (prepared inExample 6) have thermal lability. Thus, they are expected to thermallydecompose during the assay, perhaps before entering the cells or beforerelease by photolysis. Thermal decomposition of both bioconjugates wasmonitored by a UV-vis diode array spectrophotometer (HP8452) at 37° C.in water, cell-free media, and filtered media in which HCT-116 cells hadbeen grown to a concentration of about 100,000 cells/mL. Spectra weretaken hourly for a total of 8 hrs. The presence of intact bioconjugatewas then determined by photolysis, 20 min, with a high-pressure mercurylamp. If photolysis had no effect on the spectrum, all of thebioconjugate was assumed to have decomposed.

In Vitro Assays of 3 and 4 Activity

Both bioconjugates were assayed against HCT-116, HL-60, B-16, Meth-A,and RD-995 cell lines. The assays were performed in the same manner asdescribed in Example 8 as modified herein. The B-16, RD-995 and Meth-Acells lines are all Balb/c derived carcinoma lines which were providedby Dr. R. Daynes of the University of Utah. These cell lines were grownin RPMI media which was completed with 5% fetal bovine serum and othermedia components as previously described. Both the B-16 and RD-995 celllines were suspended in trypsin, as in the case of HCT-116 cells. TheMeth-A cells loosely adhere to the walls of the flask and grow bothattached to the flask and suspended in solution. These cells could becompletely suspended by successive washing of the flask wall with media.

Assays were performed at cell concentrations of about 40,000 cells perwell, with the exception of the HL-60 assay which was performed at25,000 cell per well. The HCT-116, B-16 and RD-995 cells were assayed inflat bottomed, 96-well plates, while the HL-60 and Meth-A cells wereassayed in round bottomed plates. Chlorambucil, unconjugated, was testedprior to the bioconjugates. The cells were treated with thebioconjugates in both non-photolytic and photolytic conditions. Thecells were incubated for three days (media was aspirated and replacedafter 24 h. in the case of the HL-60 cells) and the resulting viabilitymeasured by an MTT assay.

The MTT assay was somewhat altered for this experiment. The culturemedium was aspirated after 72 hrs. The Meth-A and HL-60 cells werecentrifuged prior to aspiration. Then, 100 μL of McCoy's media and 11 μLof the MTT solution were added as before. At the end of 4 hours, theculture medium was aspirated a second time (following centrifugation inthe case of HL-60 and Meth-A cells) and 100 μL of DMSO was added. TheDMSO lysed the cells releasing the MTT dye into solution. The absorbanceof each well at 450 nm was measured as before. The HCl/ethanol solutionpreviously used has a tendency to precipitate proteins from theresulting solution, which may give falsely increased absorbancemeasurements. The replacement of DMSO avoids this problem.

The concentration of chlorambucil and the bioconjugates were varied from0.04 μM to 400 μM within the assay. The cells were treated with thebioconjugates under dim, red lights to avoid photolysis. Non-photolyticconditions were maintained by wrapping the 96 well plates with foilduring the incubation periods. Photolysis was performed in black plateswith flat, clear bottomed wells (Costar catalog number: 3603). Theseplates are sterile, collagen treated, and made of optically clearplastic. Growth of the cells in these plates did not show anydifferences to those grown in the normal clear plastic plates.Photolysis was achieved by an array of high intensity green LEDs(Hewlett Packard catalog number: 782-6124). The array was constructedfrom one of the black plates in which one LED was placed in each well.The LEDs could be turned on and off as vertical rows. In each assay, tworows of cells were left untreated as growth controls. One of these rowswas not photolyzed by the LEDs to demonstrate any unexpected effects ofphotolysis; irradiation did not demonstrate any effects on the untreatedcontrol cells. An empty plate was placed between the array and the assayplate to avoid heating the cells. Ten minutes of irradiation producedcomplete photolysis for the bioconjugate in cell-free media. The cellswere irradiated for 10 min during the assay. The time of irradiation,following treatment with drug, was determined by a timecourse assay. Theentire plate was treated with one of the bioconjugates at aconcentration equal to the IC₅₀ of chlorambucil in that cell line. Therows were irradiated separately one half hour after treatment and thenhourly.

Irradiation at 1 h after treatment demonstrated the greatestbioconjugate activity in all of the cell lines. Further assays wereperformed with irradiation one hour after treatment. In the case of theMeth-A cell line, the cells were transferred from the round-bottomedplate into the black plate for photolysis and then returned to the roundbottomed plate. The HL-60 cell line could not be tested under thesephotolytic conditions.

Results and Discussion

Both bioconjugates show significant thermal decomposition in both waterand cell free media at 37° C. At the end of 8 hrs, photolysis has noeffect on the spectrum, indicating that all of the bioconjugate hasdecomposed. In the HCT-116 cell media both bioconjugates show fastinitial decomposition and are significantly stabilized at subsequenttime. Haptocorrin, a cobalamin binding protein is known to stabilizealkylcobalamins by several orders of magnitude. This protein is presentin the cell-free media from the added bovine serum. However, most ofthis protein in serum is saturated with cobalamin, so binding to thebioconjugates may be inhibited. It is known that several types of tumorcells secrete high amounts of cobalamin binding proteins, especiallyhaptocorrin. Thus, media in which cells have been growing has a higherconcentration of apo-haptocorrin. The initial fast decomposition of thebioconjugates represents the amount remaining after the saturation ofhaptocorrin in the media. The bound bioconjugates are significantlystabilized by haptocorrin and the haptocorrin complex associates anddissociates in a dynamic fashion in solution, especially in the presenceof significant concentrations of other cobalamins in the media. Thus,the bioconjugates are still susceptible to decomposition when not boundto haptocorrin. While haptocorrin does stabilize the bioconjugates byseveral orders of magnitude, slow decomposition is still seen during theassay. However, this assay does indicate that the bioconjugates arestabilized such that a significant amount is still intact during thetime of uptake and photolysis.

The data from the photolysis timecourse and activity assays aresummarized in FIGS. 5-9 for compound 3 in each of the cell lines tested.Similar results were seen for compound 4. In general, both bioconjugatesshowed similar uptake and photolysis behavior in the photolysistimecourse assay. The maximal photolytically induced toxicity is seenone hour after treatment with either of the bioconjugates. In all cases,photolysis of the bioconjugate demonstrated increased toxicity over thatof unconjugated chlorambucil. FIG. 5 shows that the chemotherapeuticdrug, chlorambucil, has an LD₅₀ of about 2 μM with respect to theHCT-116 cell line, whereas the bioconjugate shows no substantialtoxicity at concentrations approaching 100 μM. If cells treated with thebioconjugate are subjected to brief irradiation with red light 12 hoursafter dosing, the LD₅₀ decreases by a factor of 25 to 0.08 μM. If a10-fold excess of vitamin B₁₂ is added to saturate the cell surfacereceptors, the bioconjugate is not taken into the cells and photolysistriggers release of the active chlorambucil in the cell culture medium.The released chlorambucil now enters the cell by passive diffusion andan LD₅₀ of 2 μM is observed—in close agreement with the value forchlorambucil standard.

FIG. 6 shows that in cell line HL-60, the unconjugated chlorambucilstandard exhibits an LD₅₀ of 0.5 μM, but the bioconjugate is at least2-fold better with an LD₅₀ of 0.2 μM. The cytotoxicity of MC-121 againstthe leukemia cell line is still a dramatic result when compared with theabsence of toxicity when the cellular uptake of the conjugate isout-competed by the addition of 10 equivalents of vitamin B₁₂. Similarresults were obtained with Meth-A cells. The HL-60 and Meth-A cells havea high turnover rate, and in the case of Meth-A divide more rapidly thanthe other cell lines. These cells may, in fact, metabolize cobalamin ata faster rate than the other cell lines and thus release thechlorambucil in significant concentrations without photolysis. In orderfor this to be practical, however, cobalamin metabolism must occurbefore significant hydrolysis of chlorambucil moiety. It is reportedthat HL-60 cells are able to convert vitamin B₁₂ into the othercobalamin forms efficiently (more quickly than normal lymphocytes)(Quadros and Jacobsen, 1995) and thus, would be able to efficientlyrelease the conjugated chlorambucil. In the other cell lines, however,the bioconjugates are essentially not toxic in non-photolyticconditions, which is a promising indication that these bioconjugates maynot be toxic in normal somatic cells or healthy hematopoetic cells. IC₅₀values of the bioconjugates in both non-photolytic and photolyticconditions are summarized in Table 1.

TABLE 1 IC₅₀ values (μM) Cell Line HCT-116 HL-60 B-16 Meth-A Rd-995Chlorambucil 20.8 5.8 1.4 1.8 1.1 Photolysis 3 1.7 — 0.6 0.2 0.2 4 1.1 —0.3 0.3 0.3 No Photolysis 3 — 3.2 — 210.1 — 4 — 8.9 — 84.2 —

It will be appreciated that the methods and compositions of the instantinvention can be incorporated in the form of a variety of embodiments,only a few of which are disclosed herein. It will be apparent to theartisan that other embodiments exist and do not depart from the spiritof the invention. Thus, the described embodiments are illustrative andshould not be construed as restrictive.

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What is claimed is:
 1. A method of administering a bioactive agent tocells of a targeted tissue site of a subject which comprisesadministering to said subject a bioactive agent as a bioconjugate, saidbioconjugate comprises the bioactive agent and an organocobalt complexwherein the bioactive agent is covalently conjugated to the cobalt atomof the organocobalt complex through a non-reactive atom in the bioactiveagent molecule, and which cells of a targeted tissue comprise receptorsfor the organocobalt complex that mediate endocytotic activity.
 2. Themethod of claim 1, wherein the receptors of said cells of said targetedtissue site bind cobalamin.
 3. The method of claim 1, wherein said cellsof said targeted tissue site bind a targeting molecule of theorganocobalt complex portion of said bioconjugate.
 4. The method ofclaim 1, wherein said bioconjugate is administered intravenously.
 5. Themethod of claim 1, wherein said bioconjugate is administeredparenterally.
 6. The method of claim 1, wherein said bioconjugate isadministered orally.
 7. The method of claim 1, wherein said bioconjugateis administered intramuscularly.
 8. The method of claim 1, wherein saidbioconjugate is administered intrathecally.
 9. The method of claim 1,wherein said bioconjugate is administered as an aerosol.
 10. The methodof claim 1, wherein said targeted tissue site is neoplastic tissue andsaid bioactive agent is an anticancer agent.
 11. The method of claim 10,wherein said neoplastic tissue is tissue of a sarcoma.
 12. The method ofclaim 10, wherein said neoplastic tissue is tissue of a carcinoma. 13.The method of claim 10, wherein said neoplastic tissue is tissue of aleukemia.
 14. The method of claim 1, wherein said targeted tissue siteis tissue afflicted with psoriasis and said bioactive agent is acytotoxic agent or anti-metabolite.
 15. The method of claim 1, whereinsaid targeted tissue site is tissue for the application of gene therapyand said bioactive agent is an oligonucleotide or a polynucleotide. 16.The method of claim 15, wherein said oligonucleotide is antisense DNA orRNA.
 17. The method of claim 1, wherein said targeted tissue site istissue for the application of peptide therapy and said bioactive agentis a peptide or protein.
 18. The method of claim 1, wherein a bolus ofvitamin B₁₂ is administered prior to administration of saidbioconjugate.
 19. The method of claim 1, wherein nitrous oxide isadministered first to deplete body stores of vitamin B₁₂.
 20. The methodof claim 1, wherein said non-reactive atom is selected from the groupconsisting of a carbon atom, a nitrogen atom, an oxygen atom, a sulfuratom, a selenium atom or a silicon atom.
 21. The method of claim 1,wherein said non-reactive atom is a carbon atom.
 22. The method of claim1, wherein the non-reactive carbon atom is a carbon atom from an alkyl,acyl or aryl group that will not lead to rearrangement or destruction ofthe bioactive agent under conditions of ligand exchange duringreceptor-mediated endocytosis.
 23. The method of claim 1, wherein saidbioactive agent is covalently bound directly to the cobalt atom of theorganocobalt complex.
 24. The method of claim 1, wherein said bioactiveagent is covalently bound indirectly to the cobalt atom of theorganocobalt complex via a spacer.
 25. The method of claim 24, whereinsaid spacer is a self-destructing linker.
 26. The method of claim 1,wherein said bioactive agent is a diagnostic compound.
 27. The method ofclaim 1, wherein said bioactive agent is a drug.
 28. The method of claim27, wherein said bioactive agent is an anticancer agent.
 29. The methodof claim 1, wherein said bioactive agent is a peptide, peptide analogue,protein or protein analogue.
 30. The method of claim 1, wherein saidbioactive agent is a nucleic acid or a nucleic acid analogue.
 31. Themethod of claim 30, wherein said nucleic acid or nucleic acid analogueis a polynucleotide, oligonucleotide, antisense DNA or antisense RNA.32. The method of claim 1, wherein said organocobalt complex iscobalamin, cobalamin lactone, cobalamin lactam, or a cobalaminderivative, wherein said cobalamin derivative is (a) cobalamin in whichthe benzimidizaole ring is substituted with a halogen, hydroxy or a C₁₋₆alkyl, (b) an anilide, ethylamide, monocarboxylic acid, dicarboxylicacid, tricarboxylic acid or proprionamide derivative of cobalamin, or(c) cobalamin substituted with an amino, a nitro, a halogen, a sulfito,a C₂₋₆ alkylene or a C₂₋₆ alkyne.
 33. The method of claim 1, whereinsaid organocobalt complex is a compound having the following formula:

wherein R is H, amino, C₁₋₆ alcohol, or C₁₋₆ carboxyl, W, W′, X, X′, Y,Y′, Z and Z′ are independently H, amino, C₁₋₆ alcohol, C₁₋₆ carboxyl,SO₃—, CH₂OH, CO₂H, or nitro, or W and X together form a 4-6 membercyclic or heterocyclic ring, or W′ and X′ together form a 4-6 membercyclic or heterocyclic ring, or Y and Z together form a 4-6 membercyclic or heterocyclic aromatic ring or Y′ and Z′ together form a 4-6member cyclic or heterocyclic aromatic ring.
 34. The method of claim 33,which further comprises a targeting molecule covalently linked to one ofsaid R, W, W′, X, X′, Y, Y′, Z or Z, wherein said targeting molecule isselected from the group consisting of glucose, galactose, mannose,mannose 6-phosphate, transferrin, cobalamin, asialoglycoprotein,α-2-macroglobulins, insulin, a peptide growth factor, folic acid orderivatives, biotin or derivatives, YEE(GalNAcAH)₃ or derivatives,albumin, texaphyrin, metallotexaphyrin, a vitamin, a coenzyme, anantibody, an antibody fragment and a single-chain antibody variableregion (scFv).
 35. The method of claim 1, wherein said organocobaltcomplex is selected from the group consisting oforgano(pyridine)bis(dimethylglyoximato)cobalt, a corrinoid, orderivatives thereof, wherein said derivative is (a) a corrinoid in whichthe benzimidizaole ring is substituted with a halogen, hydroxy or a C₁₋₆alkyl, (b) a corrinoid substituted with an amino, a nitro, a halogen, asulfito, a C₂₋₆ alkylene or a C₂₋₆ alkyne, or (c)organo(pyridine)bis(dimethyl-glyoximato)cobalt substituted with anamino, a nitro, a halogen, a sulfito, a C₂₋₆ alkylene or a C₂₋₆ alkyne.36. The method of claim 1, wherein said organocobalt complex comprises amultiple unsaturated heterocyclic ring system bonded to a cobalt atomthrough 4-5 nitrogens and/or chalcogens which are part of said ringsystem.